Arslan, SezaÖzkardeş, Fatma2021-06-232021-06-2320070074-02761678-8060https://doi.org/10.1590/S0074-02762007000100004https://hdl.handle.net/20.500.12491/6130A total of 187 isolates from several clinical specimens were identified to species level as 129 Staphylococcus aureus strains and 58 coagulase-negative staphylococci (CNS) strains by the AN Staph System (Biomerieux). Slime production was detected both by the conventional Christensen's method as well as by the Congo red agar method. Seventy-two strains of staphylococci isolates (38.5%) were found to be slime producers by Christensen's test tube method whereas 58 strains (31%) were slime positive with Congo red agar method. There was no statistically significant difference between the two methods for the detection of slime production (P > 0.05). Susceptibility of isolates against antimicrobial agents was tested by the disk diffusion method. Staphylococcal species had resistance to one or more antibiotics. Among the various antimicrobial agents, oxacillin (71.1%) and erythromycin (47.1%) showed higher resistance than most of the agents used against all isolates. Oxacillin resistant S. aureus (ORSA) and oxacillin resistant coagulase-negative staphylococci (ORCNS), 97 (75.2%) and 36 (62.1%) respectively were frequently observed in strains isolated from clinical materials. Among the ORSA strains, two strains were resistant to vancomycin. Moreover, 96 (74.4%) of 129 S. aureus strains were positive for P-lactamase enzyme. However, 78 (81.25%) of 96 P-lactamase positive S. aureus strains were P-lactamase positive ORSA isolates, but none of them had vancomycin resistance.eninfo:eu-repo/semantics/openAccessStaphylococcus sp.SlimeAntimicrobial susceptibilityBeta-lactamaseClinical isolatesArticle10.1590/S0074-0276200700010000410212933172939952-s2.0-33847149359Q2WOS:000244101000004Q3