Erkol, Mehmet HayriKahramansoy, NurettinKordon, ÖzgürBüyükaşık, OktaySerin, ErdinçKükner, Aysel2021-06-232021-06-2320121300-49482148-5607https://doi.org/10.4318/tjg.2012.0396https://hdl.handle.net/20.500.12491/7258Background/aims: This study aimed at investigating the protective effects of beta-glucans on the lungs in obstructive jaundice. Methods: In total, five groups -Sham (1), control (2) and treatment groups (3,4,5)- were established; each comprising randomly selected seven Wistar Albino rats. Beta-glucan was given after choleduct ligation in Group 3 while it was given before and after the choleduct ligation in Group 4. As pre-treatment beta-glucan was given before ligation in Group 5. Beta-glucan was administered in a single dose of 50 mg / kg / day by gavage for a ten-day period. Superoxide dismutase, and myeloperoxidase levels in serum; malotzdialdehyde, lipid hydroxyperoxidase and glutathione levels in lung tissue; lactate dehydrogenase levels in bronchoalveolar lavage fluid were measured. Results: The blood polymorphonuclear leukocytes level was highest in the control group and lower in the sham and treatment groups. Serum superoxide dismutase and tissue glutathione values were significantly higher in Groups 3 and 4 (p <= 0.04) whilst Groups 3 and 4 did not differ from each other. In Groups 3 and 4 malondialdehyde, lipid hydroxyperoxidase, and myeloperoxidase values were significantly lower. However, Groups 3 and 4 did not differ for malondialdehyde or lipid hydroxyperoxidase values. Lactate dehydrogenase level in the bronchoalveolar lavage fluid was significantly lower in all of the treatment groups (Groups 3,4,5) (p <= 0.008). When compared to the control group, it was observed that lung damage was much more limited in the treatment groups (p<0.001). Conclusion: This study suggests that beta-glucan exhibits protective effect in pulmonary tissue against oxidative damage in obstructive jaundice.Amaç: Bu çal›flmada, beta-glukan›n deneysel t›kanma sar›l›¤› modelinde akci¤er üzerine koruyucu etkisinin araflt›r›lmas› amaçland›. Yöntem: Her biri rastgele seçilmifl yedi Wistar Albino rattan oluflan, Sham (1), kontrol (2) ve tedavi gruplar› (3,4,5) olmak üzere toplam befl grup oluflturuldu. Beta-glukan Grup 3’e koledok ligasyonundan sonra, Grup 4’e ligasyondan önce ve sonra verildi. Grup 5’e ise ön tedavi olarak ligasyondan önce verildi. Beta-glukan uygulamas›, 50 mg/kg/gün fleklinde tek doz halinde gavaj yoluyla on gün boyunca gerçeklefltirildi. Serumda süperoksit dismutaz, myeloperoksidaz; akci¤er dokusunda malondialdehit, lipid hidroperoksidaz, glutatyon; bronkoalveoler lavaj s›v›s›nda laktat dehidrogenaz ölçümleri ile histopatolojik de¤erlendirilme yap›ld›. Bulgular: Kan PNL düzeyi, kontrol grubunda en yüksek iken sham ve tedavi gruplar›nda düflük idi. Kontrol grubu ile karfl›laflt›- r›ld›¤›nda serum süperoksit dismutaz ve doku glutatyon düzeyleri Grup 3 ve 4’te belirgin yüksek iken (p≤0.04) Grup 3 ile 4 aras›nda farkl›l›k gözlenmedi. Grup 3 ve 4’te malondialdehit, lipid hidroperoksidaz, myeloperoksidaz de¤erleri belirgin düflük idi. Bununla birlikte Grup 3 ile 4 aras›nda malondialdehit ve lipid hidroperoksidaz de¤erleri farkl› de¤ildi. Bronkoalveoler lavaj s›v›s›ndaki LDL de¤erleri tüm tedavi gruplar›nda (Grup 3,4,5) anlaml› derecede düflük idi (p≤0.008). Kontrol grubu ile karfl›laflt›r›ld›¤›nda akci¤er hasar›n›n tedavi gruplar›nda oldukça s›n›rl› oldu¤u tespit edildi (p<0.001). Sonuç: Bu çal›flma, t›kanma sar›l›¤›nda betaglukan›n akci¤er dokusunda oksidatif hasara karfl› koruyucu etkiye sahip oldu¤unu göstermektedir.eninfo:eu-repo/semantics/closedAccessObstructive JaundiceBeta-GlucanLung DamageOxidative DamageAntioxidantsTıkanma SarılığıBeta-GlukanAkciğer HasarıOksidatif HasarAntioksidanlarArticle10.4318/tjg.2012.03962313845225053782-s2.0-84859595366Q3132551WOS:000302825900007Q4