Dinç, ErdemAyaz, LokmanKurt, Akif HakanDursun, ÖzerYılmaz, GülşenVatansever, Mustafa2023-07-032023-07-032021Dinç, E., Ayaz, L., Kurt, A. H., Dursun, Ö., Yılmaz, G., Vatansever, M., ... & Yılmaz, Ş. N. (2021). Effects of bone marrow and adipose-derived mesenchymal stem cells on microRNA expressions in acute alkaline corneal burn. Journal of Ocular Pharmacology and Therapeutics, 37(4), 200-208.1080-76831557-7732http://dx.doi.org/10.1089/jop.2020.0107https://hdl.handle.net/20.500.12491/11226Purpose: The aim of this study was to investigate the microRNA (miRNA) expressions of the corneal tissue after an alkaline burn and to compare the efficiency of adipose- and bone marrow-derived mesenchymal stem cells (MSCs) on expressions. Methods: Thirty-two rats were divided into 4 groups. No intervention was made in the control group. A chemical burn was created by applying 4 mu L NaOH soaked in 6 mm filter paper to the right eye of each animal in the other groups. Whereas only subconjunctival 0.1 mL phosphate-buffered saline (PBS) was injected to in the group 1, 2 x 10(6) adipose- or bone marrow-derived MSC in 0.1 mL PBS was injected subconjunctivally to the animals in the remaining groups (groups 2 and 3, respectively). Tissue samples were collected for miRNA analysis on the third day after the burn. Results: When group 1 was compared with the control group, the expression of 3 of 93 miRNAs increased significantly, whereas the expression of 50 miRNAs decreased significantly. Significant changes in miRNA expressions were observed when group 1 was compared with groups 2 and 3. Although a significant change was observed in the expression of 6 miRNAs in the adipose-derived MSC group, it was found that the expression of 65 miRNAs significantly changed in the bone marrow-derived MSC group. Conclusion: This study shows that there are significant changes in some miRNA expressions after corneal alkaline burn and these changes can be reversed with the subconjunctival injection of MSCs.eninfo:eu-repo/semantics/closedAccessAlkaline Corneal BurnMesenchymal Stem CellMirnaChemical BurnPBSArticle10.1089/jop.2020.0107374200208334816572-s2.0-85105719007Q2WOS:000648085500003Q3