Uzun, ÖzgeDemiryürek, A. Tuncay2021-06-232021-06-2320030014-2999https://doi.org/10.1016/S0014-2999(02)02761-9https://hdl.handle.net/20.500.12491/5519The aim of this study was to determine the role of nuclear factor-kappaB (NF-kappaB) in hypoxic constriction of isolated pulmonary arteries. Rings were suspended in an organ bath filled with Krebs-Henseleit solution and isometric contractions were recorded continuously. Hypoxia (%95 N-2-%5 CO2) had no marked effect on resting force in artery rings. However, hypoxia caused further contractions in serotonin-precontracted arteries. Hypoxia-induced vasoconstrictions were abolished by preincubation with NF-kappaB inhibitors, pyrrolidine dithiocarbamate (100 muM) or pyrithione (10 muM). These results suggest that reactive oxygen species and/or NF-kappaB activation may be involved in the hypoxia-induced vasoconstriction in sheep-isolated pulmonary arteries.eninfo:eu-repo/semantics/closedAccessHypoxiaPulmonary ArterySheepPyrrolidine DithiocarbamatePyrithioneArticle10.1016/S0014-2999(02)02761-94581-2171174124989222-s2.0-0037222451Q1WOS:000180303500022Q2