Çetinkaya, K.Atasever, MelahatErişgin, ZüleyhaSönmez, ÇiğdemAlışık, MuratÖzer, Çiğdem2024-05-242024-05-242023Cetinkaya, K., Atasever, M., Erisgin, Z., Sonmez, C., Ozer, C., Coskun, B., & Alisik, M. (2023). The role of oxidative stress in chemotherapyinduced gonadotoxicity in a rat model, and the protective effects of Nigella Sativa oil on oxidative stress, the anti-Müllerian hormone level, and apoptosis. European Review for Medical & Pharmacological Sciences, 27(13).1128-3602https://www.webofscience.com/wos/woscc/full-record/WOS:001045159300009PUBMED ID: 37458651https://hdl.handle.net/20.500.12491/12162This study was supported by Scientific Research Project, Giresun University, Turkey (Project grant number: SAG-BAP-A-140316-95).OBJECTIVE: This study aimed to determine the role of oxidative stress (OS) in carboplatin-induced gonadotoxicity and whether Nigella Sativa oil (NSO), an herbal antioxidant, has a protective effect on ovarian apoptosis, OS, and the anti-Mullerian hormone (AMH) level in a rat model. MATERIALS AND METHODS: The study included 24 adult female rats that were divided into 4 treatment groups. Group A saline + saline (sham group); group B: NSO + saline; group C: saline + carboplatin; group D: NSO + carboplatin. Saline, NSO, and carboplatin were administered intraperitoneally 24 and/or 48 h before sacrification as 4 mL/kg, 4 mL/kg, and 80 mg/kg, respectively. Apoptosis, OS parameters, and AMH were measured. RESULTS: Oxidant levels and apoptosis were higher, whereas AMH and the antioxidants were lower in group C than in group A. Apoptosis, OS parameters, and AMH levels were negatively affected by chemotherapy (CTx) in group C whilst improvement in those parameters was observed in group D following NSO pretreatment. The levels of apoptosis and malondialdehyde (MDA), an OS parameter, in group D were lower than in group C as they declined from 34.3% to 8.65% (p = 0.002) and from 199.4 nmol/g tissue to 136.4 nmol/g tissue (p = 0.002), respectively. However, the slight increase in AMH level from 2.7 ng/mL to 3.5 ng/mL due to the NSO effect was not significant between groups C and D. CONCLUSIONS: The present findings show that carboplatin has adverse effects on AMH, ovarian tissue apoptosis, and OS parameters. NSO pretreatment might protect ovarian tissue and decrease CTx-induced ovarian injury by decreasing OS and apoptosis, but the protective effect of NSO on AMH is limited.eninfo:eu-repo/semantics/closedAccessAntioxidantApoptosisGonadotoxicityNigella Sativa OilOxidative StressOvarian ReserveArticle271363436350#YOKQ2WOS:001045159300009N/A