Gillis, Richard B.Adams, Gary G.Wolf, BettinaBerry, MonicaBesong, Tabot M. D.Kök, Şamil Mehmet2021-06-232021-06-2320130144-86171879-1344https://doi.org/10.1016/j.carbpol.2012.05.018https://hdl.handle.net/20.500.12491/76222nd International Polysaccharide Conference on Polysaccharides as Source of Advanced and Sustainable Products (EPNOE) -- AUG 29-SEP 02, 2011 -- Wageningen, NETHERLANDSMucins are the key macromolecular component of mucus, nature's natural lubricant, and one of the most important physical properties is their molecular weight distribution. A new approach for polydisperse polymers was recently published based on sedimentation velocity in the analytical ultracentrifuge and converts a distribution of sedimentation coefficient g(s) vs. s plot into a distribution of molecular weight utilising the power-law or scaling relationship between the sedimentation coefficient and molecular weight, s = kappa(s) M-w(b) where s is the sedimentation coefficient, M-w, is the weight average molecular weight and kappa(s) and bare characteristic coefficients related to conformation. We investigate the possibility of using a large database of previously published values of s an M to define kappa(s) and b for both aqueous solution and aqueous solution supplemented by 6 M guanidine hydrochloride (a solvent which helps to minimise sample degradation). These values are then applied to a study of the molecular weight distributions of preparations of human gastric mucin in the different solvents and at different stages of purification. (C) 2012 Elsevier Ltd. All rights reserved.eninfo:eu-repo/semantics/closedAccessMucinsExtended Fujita ApproachColumn and Membrane FreeConference Object10.1016/j.carbpol.2012.05.018931178183234659172-s2.0-84874648515Q1WOS:000316512900025Q1