Öztürk, HayrettinTuncer, Mehmet CudiÖztürk, HülyaBüyükbayram, Hüseyin2021-06-232021-06-2320070886-022X1525-6049https://doi.org/10.1080/08860220601166289https://hdl.handle.net/20.500.12491/6173This study was designed to determine the effect of L-arginine on hypoxia inducible factor alpha (HIF-1 alpha) and Sonic hedgehog (Shh) levels considered to be involved in the development of ischemia/reperfusion (I/R) injury. Unilaterally nephrectomized Sprague-Dawley rats were subjected to 60 minutes of left renal ischeMia followed by 45 minutes of reperfusion. Group I were sham-operated animals; group 2, I-R/Untreated animals; and group 3, I-R/L-Arg-treated animals. Serum creatinine, blood urea nitrogen (BUN), and kidney malondialdehyde (MDA eve were determined as well as examining the kidneys histologicall The treatment of rats with L-Arg produced a significant reduction in the levels of BUN, creatinine, MDA, and histopathological score compared to renal I/R groups. The Shh expression in the tubulus epithelia were intensely increased in the I-R/L-Arg group when compared to that of the Sham-control and the I-R/ untreated groups. Additionally, the HIF-fix expression in the tubulus epithelia and the interstitial spaces were intensely increased in the I-R/L-Arg group. These findings suggest that NO reduces the renal dysfunction associated with I/R of the kidney and may act as a trigger to induce Shh and HIF-1 activity.eninfo:eu-repo/semantics/openAccessRenal/KidneyIschemia/Reperfusion InjuryHypoxia Inducible Factor Alpha (HIF-1a)Sonic Hedgehog (Shh)Article10.1080/08860220601166289293249256174974362-s2.0-34248512606Q2WOS:000246884800001Q4