Dede, GizemSaylan, Aslıhan2024-03-142024-03-142022Dede, G., & Saylan, A. (2022). The effect of astaxanthin on human sperm parameters after cryopreservation. Canadian Urological Association Journal, 16(11), E552.1920-1214http://dx.doi.org/10.5489/cuaj.7876https://hdl.handle.net/20.500.12491/12086Introduction: The aim of the study was to examine the protecti-ve efficacy of astaxanthin (ASTA) against the damage that occurs during sperm cryopreservation.Methods: This experimental study was carried out on waste semen samples of 30 normozoospermic individuals who applied for semen analysis. Semen samples were divided into four equal volumes and 0 pM (control group), 50 pM, 100 pM, and 500 pM ASTA were added to each group. All groups were stored frozen in a liquid nitrogen tank. Semen samples were removed from liquid nitrogen after 72 hours and were thawed. Motility evaluation of sperm was performed. In addition, sperm was stained with acidic aniline blue to detect DNA chromatin condensation.Results: The highest motility loss was found in the control group and the least motility loss was in the 100 pM ASTA group. When examined in terms of sperm chromatin condensation, condensed sperm count was higher in the 100 pM ASTA group than in the other groups.Conclusions: It has been observed that ASTA added to the cryopro-tectant substance during sperm cryopreservation positively affects sperm motility and reduces the number of decondensed sperm.eninfo:eu-repo/semantics/openAccessOxidative StressSpermatozoaMorphologyQualityCellsArticle10.5489/cuaj.78761611E552E557357049362-s2.0-85133062832N/AWOS:000882962100008Q4