Akoğlu, AylinEngin, DorukÇakmakçı, M. Lütfü2021-06-232021-06-2320150379-51360975-1033https://hdl.handle.net/20.500.12491/8384http://nopr.niscair.res.in/bitstream/123456789/34734/1/IJMS%2044%284%29%20539-545.pdfSome properties of C-Phycocyanin (C-PC) from a native strain Anabaena affinis isolated from Kovada Lake, Isparta, Turkey were discussed in the present. Purification process was resulted in analytical grade product with 6.93-fold increase in phycocyanin purity. By using UV-VIS and fluorescence spectrophotometry, the highest absorbance and fluorescence were determined as 620 and 646 nm, respectively. SDS-PAGE analysis revealed the presence of two major bands with a molecular mass of 17.1 and 18.6 kDa corresponding to the a and 13 subunits and as confirmed by MALDI-TOF mass spectrometric analysis. Molecular mass of pure C-PC was found as 156.2 and 198 kDa by HPLC gel filtration. Aggregation states of C-PC were estimated as hexameric-(alpha beta)(6) and trimeric forms-(alpha beta)(3) along with two binding polypeptides of 27 kDa. High antioxidant activity was determined at 480 mu M TEAC/mg phycocyanin while no antimicrobial activity was detected.eninfo:eu-repo/semantics/openAccessPhycocyaninCyanobacteriaAnabaena AffinisPurificationAntioxidant ActivityArticle4445395452-s2.0-84978926937N/AWOS:000378978200013Q4