Study on an Hydrophilic Interaction Electrochromatography Method for Separation of Sulfonamide Antibiotics

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dc.contributor.authorAydoğan, Cemil
dc.contributor.authorYılmaz, Fatma
dc.contributor.authorÇimen, Duygu
dc.contributor.authorAndaç, Müge
dc.contributor.authorShaikh, Huma
dc.contributor.authorDenizli, Adil
dc.date.accessioned2024-09-25T20:19:54Z
dc.date.available2024-09-25T20:19:54Z
dc.date.issued2014
dc.departmentAbant İzzet Baysal Üniversitesien_US
dc.description.abstractThis study describes the preparation of a hydrophilic monolithic column and its application to sulfonamide antibiotics. The column was prepared by single step in situ polymerization of 2-hydroxyethyl methacrylate (HEMA), ethylene dimethacrylate (EDMA) and methacrylic acid (MAA) in a binary porogenic solvent consisting of toluene and 1-dodecanol, inside a 100 µm-i.d. capillary. The resulting monolith was electrochromatographically characterized as well as SEM. The prepared column showed hydrophilic behaviour using thiourea and toluene as markers. The SEM images showed that the monolithic column composed of spherical particles of approximately 2 µm in diameter. Using this hydrophilic monolith as stationary phase, hydrophilic interaction electrochromatography of sulfonamide antibiotics as a new method was developed. The method was successfully used for the separation of sulfonamide antibiotics. Some parameters including acetonitrile (ACN) content, pH and ionic strength on the separation of the sulfonamides, namely sulfaprydine, sulfadiazine, sulfamethazine, sulfisoxazole and sulfadoxine were also investigated. A typical hydrophilic interaction separation mechanism was revealed at higher organic solvent content (ACN > 60%).en_US
dc.identifier.endpage450en_US
dc.identifier.issn1303-5002
dc.identifier.issn2687-475X
dc.identifier.issue3en_US
dc.identifier.startpage443en_US
dc.identifier.trdizinid489917en_US
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/489917
dc.identifier.urihttps://hdl.handle.net/20.500.12491/15827
dc.identifier.volume42en_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.relation.ispartofHacettepe Journal of Biology and Chemistryen_US
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmzYK_20240925en_US
dc.titleStudy on an Hydrophilic Interaction Electrochromatography Method for Separation of Sulfonamide Antibioticsen_US
dc.typeArticleen_US

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