Biofilm formation, siderophore production, virulence-associated genes and genetic diversity of Yersinia enterocolitica isolated from foods

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Küçük Resim

Tarih

2022

Dergi Başlığı

Dergi ISSN

Cilt Başlığı

Yayıncı

Hellenic Veterinary Medical Soc

Erişim Hakkı

info:eu-repo/semantics/openAccess

Özet

Yersinia enterocolitica is a foodborne pathogen widely found in nature and foods. The ability of this organism to multiply at refrigeration temperatures in food products with a prolonged shelf-life renders it a potential health risk for consumers. Y. enterocolitica isolates from foods were investigated for biofilm formation and siderophore production at different temperatures (12, 25 and 37 ??C) using the microtiter plate and CAS agar plate meth-ods, respectively. The isolates were also evaluated with PCR for the presence of virulence genes and rep-PCR for genetic diversity. Most of the isolates showed high capability to form biofilm at all temperatures after 24 and 48 h of incubation. The results at 12 and 25 ??C (P < 0.05) as well as at 25 and 37 ??C (P 0.05) showed a statistically significant difference in quantification of biofilm formation after 24 h; however, no difference was observed between findings at 12 and 37 ??C (P 0.05) after 48 h of incubation; conversely, no significant difference was observed between 12 and 25 ??C (P 0.05). Strong biofilm-producing isolates at 12 ??C were more prevalent at 48 h than at 24 h. Siderophore production was observed in 83.3% and 100% of the isolates at 25 and 37 ??C, respectively. However, no isolate produced siderophores at 12 ??C. Genes myfA, fepA, fepD, fes, hreP, tccC and ymoA were detected in 16.7%, 33.3%, 100%, 100%, 44.4%, 5.6%, and 33.3% of the isolates, respectively. The rep-PCR revealed a high level of genetic differentiation among the isolates. The results indicate that foods of animal origin may act as a potential vehicle for dissemination in the community of Y. enterocolitica strains producing biofilm and siderophore and carrying virulence-associated genes.

Açıklama

Anahtar Kelimeler

Yersinia Enterocolitica, Biofilms, Virulence Markers, Biotype 1a, Cold Chain, Prevalence

Kaynak

Journal of the Hellenic Veterinary Medical Society

WoS Q Değeri

Q4

Scopus Q Değeri

Q3

Cilt

73

Sayı

2

Künye

Özdemir, F., & Arslan, S. (2022). Biofilm formation, siderophore production, virulence-associated genes and genetic diversity of Yersinia enterocolitica from food. Journal of the Hellenic Veterinary Medical Society, 73(2), 4077-4084.