Mechanism of inhibition of purified leaping mullet (Liza saliens) NADPH-Cytochrome P450 reductase by toxic metals: Aluminum and thallium
Yükleniyor...
Tarih
2007
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Wiley
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Aluminum and thallium may reach life-threatening levels in aquatic systems in the near future because of their extensive use in various industrial fields. It is therefore important to study the mechanism of toxicity of aluminum and thallium on fish enzymes. To this aim, the effects of aluminum and thallium on the activity of purified leaping mullet (Liza saliens) cytochrome P450 reductase, an essential component of the important cytochrome P450 system, have been studied. Results indicated that both metal ions strongly inhibited the NADPH-cytochrome P450 reductase. The IC50 values of AlCl3 and TlCl3 were estimated to be 34 mu M and 3 mu M. respectively. The Lineweaver-Burk plot and Dixon plot revealed that both metal ions noncompetitively inhibited the purified mullet cytochrome P450 reductase. The Ki values of Al3+ and Tl3+ were calculated from Dixon plots as 8.9 and 5.6 mu M, respectively. The inhibitory effects of Al3+ and Tl3+ on purified cytochrome P450 reductase were partially recovered by 1 mM EDTA. Additionally, tin and magnesium were shown to have no apparent effect on purified mullet cytochrome P450 reductase. (c) 2007 Wiley Periodicals, Inc.
Açıklama
Anahtar Kelimeler
Aluminum, Thallium
Kaynak
Journal Of Biochemical And Molecular Toxicology
WoS Q Değeri
Q3
Scopus Q Değeri
Q2
Cilt
21
Sayı
6
