Evaluation of the expression and proliferation of degenerative markers in primary cell cultures obtained from human intervertebral disc tissue

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dc.contributor.authorKaya, Yasin Emre
dc.contributor.authorAkalan, Hande
dc.contributor.authorYılmaz, İbrahi?m
dc.contributor.authorKaraarslan, Numan
dc.contributor.authorŞirin, Duygu Yaşar
dc.contributor.authorÖzbek, Hanefi
dc.date.accessioned2021-06-23T18:24:55Z
dc.date.available2021-06-23T18:24:55Z
dc.date.issued2020
dc.departmentBAİBÜ, Tıp Fakültesi, Cerrahi Tıp Bilimleri Bölümüen_US
dc.description.abstractAim: A major cause of low back pain is disc degeneration. Nevertheless, no specific and reliable markers of the degeneration of the nucleus pulposus (NP) are available. This presented study aimed to examine changes in the expressions of genes in primary cell cultures isolated from intact and degenerated tissues to give insights into the biopathogenesis of intervertebral disc (IVD) tissue. Material and Methods: Tissues of eight patients (n = 8; average age: 41.74 ± 9.86 years) were resected through microdiscectomy, and primary cell cultures were prepared using degenerated disc tissue. The cultured degenerated tissues served as the study group. The samples in the control group comprised the intact tissues of patients (n = 8; average age: 38.68 ± 7.91 years) resected following a trauma. Morphology of the cell surface were evaluated using an inverted light/fluorescent microscopy at 0 and 24 h on days 10 and 21. The expressions of the chondroadherin (CHAD), cartilage oligomeric matrix protein (COMP), interleukin-1 beta (IL-1 beta), and matrix metalloproteinase (MMP)-7 and MMP-19 genes were evaluated using the reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The data obtained were statistically analyzed. Results: The four genes investigated, except COMP (P > 0.05), changed significantly in primary cell cultures isolated from degenerative IVD tissues. This result was statistically significant (P < 0.05). The gene expressions in the samples derived from intact IVD tissues changed markedly and these changes were associated with proliferation (P < 0.05). Conclusion: Analyzing the changes in gene expression levels associated with IVD should contribute to future studies on the prevention and treatment of such pathologies. The data obtained from the present study will shed light on cellular-based personal targeted therapies through which genetic information can be transmitted to cells.en_US
dc.identifier.doi10.5455/annalsmedres.2020.02.171
dc.identifier.endpage716en_US
dc.identifier.issn2636-7688
dc.identifier.issn2636-7688
dc.identifier.issue3en_US
dc.identifier.startpage711en_US
dc.identifier.trdizinid366957en_US
dc.identifier.urihttps://doi.org/10.5455/annalsmedres.2020.02.171
dc.identifier.urihttps://app.trdizin.gov.tr/makale/TXpZMk9UVTNOdz09
dc.identifier.urihttps://hdl.handle.net/20.500.12491/1026
dc.identifier.volume27en_US
dc.indekslendigikaynakTR-Dizinen_US
dc.institutionauthorKaya, Yasin Emre
dc.language.isoenen_US
dc.relation.ispartofAnnals of Medical Researchen_US
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subject[No Keywords]en_US
dc.titleEvaluation of the expression and proliferation of degenerative markers in primary cell cultures obtained from human intervertebral disc tissueen_US
dc.typeArticleen_US

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