De novo transcriptome assembly reveals three alternative oxidase encoding genes in Nymphaea alba L.

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dc.contributor.authorÜnlü, Ercan
dc.contributor.authorYildiz, Gulgez Gokce
dc.date.accessioned2024-09-25T20:18:11Z
dc.date.available2024-09-25T20:18:11Z
dc.date.issued2022
dc.departmentAbant İzzet Baysal Üniversitesien_US
dc.description.abstractWater lilies are aquatic, ornamental and economically valuable plants classified under Nymphaea genus. Nymphaea alba L., white water lily, has a special focus since it is a member of basal angiosperms. Alternative oxidase (AOX) proteins are the terminal oxidases in the electron transport chain of plants. Identification of alternative oxidase encoding genes for basal angiosperms is important to increase the quality of phylogenetic studies. However, AOX encoding genes were yet to be discovered for N. alba. In this study, we aimed to identify alternative oxidase encoding genes in N. alba by performing transcriptome analysis. Annotation of 272,934 unigenes with Trinotate tool revealed 77 transcripts with AOX domains characterized in known alternative oxidases. Blast analysis of these 77 sequences with known AOX proteins revealed three distinct AOX genes (AOX1, AOX2 and AOX4) in N. alba. After in silico subcellular localization analysis of three identified AOX proteins, AOX1, AOX2 are predicted as mitochondrial while AOX4 is a plastidic alternative oxidase protein. Template-based structural modeling results showed that all identified proteins are statistically similar to known structure models of corresponding AOXs.en_US
dc.identifier.doi10.29130/dubited.914845
dc.identifier.endpage299en_US
dc.identifier.issn2148-2446
dc.identifier.issue1en_US
dc.identifier.startpage285en_US
dc.identifier.trdizinid1123730en_US
dc.identifier.urihttps://doi.org/10.29130/dubited.914845
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1123730
dc.identifier.urihttps://hdl.handle.net/20.500.12491/15509
dc.identifier.volume10en_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.relation.ispartofDüzce Üniversitesi Bilim ve Teknoloji Dergisien_US
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmzYK_20240925en_US
dc.subjectAOXen_US
dc.subjectAbant Lakeen_US
dc.subjectRNA-Seqen_US
dc.subjectWater lilies.en_US
dc.titleDe novo transcriptome assembly reveals three alternative oxidase encoding genes in Nymphaea alba L.en_US
dc.typeArticleen_US

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