A fast protein liquid chromatography method for purification of myoglobin from different species

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dc.contributor.authorSarkarati, Bahram
dc.contributor.authorMilli, Nursel Söylemez
dc.contributor.authorEren, Ömer
dc.date.accessioned2024-09-25T20:23:54Z
dc.date.available2024-09-25T20:23:54Z
dc.date.issued2023
dc.departmentAbant İzzet Baysal Üniversitesien_US
dc.description.abstractThe aim of this study is to describe a fast method for the purification of high-purity myoglobin for Mass Spectroscopy analyses and to use it as standard-grade material. A three-step Fast Protein Liquid Chromatography (FPLC) method was used to produce high-purity myoglobin. SEC 650 gel filtration followed by an Enrich Q anion exchange chromatography was used to produce myoglobin in acceptable purity for most research methods. A second filtration step was carried out by narrow field SEC 70 gel to prepare high-purity myoglobin at standard grade purity and capable of Mass Spectroscopy analyses. At least 90% pure myoglobin was obtained by applying two chromatography steps in the samples of three species, and over 99% pure myoglobin was obtained in standard material quality and suitable for mass spectroscopy when the additional narrow field SEC 70 chromatography step was applied. The proposed method provides higher purity compared to other methods and can be applied in a shorter time. FPLC columns significantly reduce the duration of the chromatography steps. At the same time, the use of solid extraction columns instead of dialysis reduced the long overnight dialysis process to a few minutes.en_US
dc.identifier.doi10.24880/maeuvfd.1230538
dc.identifier.endpage103en_US
dc.identifier.issn2458-9268
dc.identifier.issn2148-6239
dc.identifier.issue2en_US
dc.identifier.startpage95en_US
dc.identifier.trdizinid1195990en_US
dc.identifier.urihttps://doi.org/10.24880/maeuvfd.1230538
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1195990
dc.identifier.urihttps://hdl.handle.net/20.500.12491/16199
dc.identifier.volume8en_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.relation.ispartofMehmet Akif Ersoy Üniversitesi Veteriner Fakültesi Dergisien_US
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmzYK_20240925en_US
dc.subjectMyoglobinen_US
dc.subjectFPLCen_US
dc.subjectprotein purificationen_US
dc.subjectspecies identificationen_US
dc.titleA fast protein liquid chromatography method for purification of myoglobin from different speciesen_US
dc.typeArticleen_US

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