Development of a Competent and Trouble Free DNA Isolation Protocol for Downstream Genetic Analyses in Glycine Species

Basit Öğe Kaydı
dc.contributor.authorNawaz, Muhammad Amjad
dc.contributor.authorBaloch, Faheem Shehzad
dc.contributor.authorRehman, Hafiz Mamoon
dc.contributor.authorLe, Bao
dc.contributor.authorAkther, Fahima
dc.contributor.authorYang, Seung Hwan
dc.contributor.authorChung, Gyuhwa
dc.date.accessioned2024-09-25T20:12:31Z
dc.date.available2024-09-25T20:12:31Z
dc.date.issued2016
dc.departmentAbant İzzet Baysal Üniversitesien_US
dc.description.abstractExtraction of deoxyribose nucleic acid (DNA) from plants is preliminary step in molecular biology. Fast and cost effective genomic DNA isolation from Glycine species for downstream application is a major bottleneck. Here we report a high throughput and trouble free method for genomic DNA extraction from leaf and seeds of Glycine species with high quality and quantity. Protocol reports the optimization by employing different concentrations of CTAB and PVP in extraction buffer. Efficiency of optimized protocol was compared with frequently used DNA extraction methods. Wide adoptability and utility of this protocol was confirmed by DNA extraction from leaves as well as seeds of G. max, G. soja, G. tomentella and G. latifolia. Extracted DNA was successfully subjected to PCR amplification of five microsatellite markers and four putative glycosyltransferase genes. DNA extraction protocol is reproducible, trouble free, rapid and can be adopted for plant molecular biology applications.en_US
dc.identifier.endpage6en_US
dc.identifier.issn2148-127X
dc.identifier.issue8en_US
dc.identifier.startpage1en_US
dc.identifier.trdizinid203508en_US
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/203508
dc.identifier.urihttps://hdl.handle.net/20.500.12491/14494
dc.identifier.volume4en_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.relation.ispartofTürk Tarım - Gıda Bilim ve Teknoloji dergisien_US
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmzYK_20240925en_US
dc.titleDevelopment of a Competent and Trouble Free DNA Isolation Protocol for Downstream Genetic Analyses in Glycine Speciesen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

TR-Dizin İndeksli Yayınlar Koleksiyonu