Effects of nutrient media strength on cardiotonic glycoside accumulation in Digitalis lamarckii Ivan, an endemic medicinal species
Küçük Resim Yok
Tarih
2011
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Georg Thieme Verlag Kg
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
The effects of different strengths of Murashige and Skoog (MS) (1) medium on cardiotonic glycoside accumulation in Digitalis lamarckii Ivan were investigated. D. lamarckii, commonly known as dwarf foxglove (yüksükotu in Turkish), is an Turkish endemic medicinal plant and belongs to the family of Plantaginaceae. The Digitalis species are biennial or perennial herbs containing important cardioactive compounds (glycosides) that are used to treat heart problems. D. lamarckii has been marked as vulnerable (VU) in the Red Data Book of Turkish Plants (2). Contents of five different cardenolides (namely, digoxigenin, gitoxigenin, lanatoside C, digoxin and digitoxin) in the callus developed from hypocotyl explants, which were cultured for 10 days on different strengths of MS media supplemented with %3 sucrose, 0.5 ppm TDZ and 0.25 ppm IAA, were determined by HPLC. Three different strenghts of MS media were tested; quarter-, half- and full-strength. Of the five cardenolides, only lanatoside C could be detected in the callus cultured on all medium strenghts. The highest concentration of lanatoside C was observed on half-strength MS medium (690µg/g dry weight, dw), while the callus cultured on full- and quarter-strength media producing 231 and 332µg/g dw lanatoside C, respectively. In conclusion, the protocol described here is expected to have an important contribution to the future efforts for a large scale production of cardenolides in Digitalis species.
Açıklama
59th International Congress and Annual Meeting of the Society-for-Medicinal-Plant-and-Natural-Product-Research -- SEP 04-09, 2011 -- Antalya, TURKEY
Anahtar Kelimeler
Cardenolide Accumulation, Digitalis Lamarckii, Medium Strength, Murashige and Skoog Basal Medium
Kaynak
Planta Medica
WoS Q Değeri
Q2
Scopus Q Değeri
Cilt
77
Sayı
12
