Determination of DNA and its binding to protamine using potentiometric polyion sensors
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Dosyalar
Tarih
2007
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Polyion-sensitive membrane electrodes based on the ion-exchanger dinonylnaphthalene sulfonate (DNNS) have previously been developed and used as an end-point detector for the titrimetric determination of heparin, low molecular weight heparins, and pentosan polysulfate (PPS). Herein, we report a similar detection methodology for double stranded DNA (dsDNA (500 bp) and dsDNA (100 bp)) in buffered solution at mu g/mL levels using protamine as a titrant. Further, it is shown that the titration data can be used to estimate the binding constants between protamine and dsDNA. The equilibrium constant K-eq and the number of protamine binding sites (n) per mole of DNA are determined by recasting the data in the form of a Scatchard plot. (c) 2006 Elsevier B.V. All rights reserved.
Açıklama
Anahtar Kelimeler
DNA determination, Polyion-sensitive electrode, Protamine, Scatchard plot
Kaynak
Journal Of Electroanalytical Chemistry
WoS Q Değeri
Q2
Scopus Q Değeri
N/A
Cilt
602
Sayı
1
