In vitro adventitious plant regeneration of Echium orientale L., an endemic plant: the evaluation of biological activities and phenolic content
Yükleniyor...
Dosyalar
Tarih
2018
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Natl Inst Science Communication-Niscair
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Echium orientale L. is a Turkish endemic plant in the family Boraginaceae. The aim of this study was to develop an efficient in vitro culture protocol for E. orientale and compare the field-grown and in vitro-grown leaves in terms of antibacterial, antitumor and antioxidant activities and phenolic profiles. Leaf and petiole explants were cultured on Murashige & Skoog (MS) medium with various plant growth regulator combinations. Best shoot proliferation was obtained from leaf explant with 1 mg/L thidiazuron (TDZ) and 0.5 mg/L indole-3-acetic acid (IAA), producing 8.7 shoots per explant at 100% shoot frequency. Regenerated shoots were transferred to rooting media containing different levels of IAA, indole-3-butyric acid (IBA), naphthalene acetic acid (NAA) or 2,4 dichlorophenoxyacetic acid (2,4-D). The best root formation was observed with MS basal medium (control). Rooted explants were transferred to vermiculate in Magenta containers for acclimatization and after 3 weeks they were transferred to plastic pots containing potting soil and maintained in the plant growth room. Leaves from field-grown plants showed higher antibacterial, antitumor and antioxidant activities, and phenolic content than those from in vitro-grown plants.
Açıklama
Anahtar Kelimeler
Antibacterial, Antioxidant, Antitumor, Echium Orientale, HPLC, In Vitro Culture, Micropropagation
Kaynak
Indian Journal Of Biochemistry & Biophysics
WoS Q Değeri
Q4
Scopus Q Değeri
Q4
Cilt
55
Sayı
4
