Bioproduction of xylitol from chestnut shell hydrolysate: Optimization of fermentation parameters

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Küçük Resim

Tarih

2019

Dergi Başlığı

Dergi ISSN

Cilt Başlığı

Yayıncı

Chiang Mai Univ, Fac Science

Erişim Hakkı

info:eu-repo/semantics/closedAccess

Özet

In this study, xylitol production of Candida tropicalis M2 was optimized in chestnut shell hemicellulosic hydrolysate. Initial xylose concentration, initial concentration of ammonium chloride which was chosen as a nitrogen source and shaking rate were selected as the independent variables. Modelling the effects of the independent variables on maximum xylitol concentration (Y-1), xylitol yield (Y-2), maximum xylitol production rate (Y-3), percentage of substrate conversion (Y-4) and specific product formation rate (Y-5) were performed by response surface methodology using central composite design. Variations of the responses with the independent variables were defined by a quadratic model except the Y-4. According to ANOVA results, initial concentration of ammonium chloride was found to be the most important parameter. Linear effect of the initial xylose concentration was found as significant for only Y-1. The effect of shaking rate was very significant for the Y-1 and Y-4. By numeric optimization conducted; the conditions with 80 g/L initial xylose concentration, 3.761 g/L initial ammonium chloride concentration and 80 strokes/min of shaking rate were predicted as optimum conditions. In optimum conditions; Y-1, Y-2, Y-3, Y-4 and Y-5 were obtained as; 24.50 g/L, 31.517%, 0.497 g/L.h, 97.35% and 0.06 g/L.h.N, respectively.

Açıklama

Anahtar Kelimeler

Xylitol, Chestnut Shell, Hemicellulosic Hydrolysate, Candida Tropicalis, Response Surface Methodology, Optimization

Kaynak

Chiang Mai Journal Of Science

WoS Q Değeri

Q4

Scopus Q Değeri

Q4

Cilt

46

Sayı

5

Künye