A STUDY ON THE EFFECTS OF DIRECT FACTOR XA INHIBITORS AND DIRECT THROMBIN INHIBITORS ON HUMAN PRIMARY CHONDROCYTE CULTURES

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dc.contributor.authorKaya, Yasin Emre
dc.contributor.authorAkalan, Hande
dc.contributor.authorYilmaz, Ibrahim
dc.contributor.authorKaraarslan, Numan
dc.contributor.authorŞirin, Duygu Yaşar
dc.contributor.authorÖzbek, Hanefi
dc.contributor.authorAteş, Özkan
dc.date.accessioned2024-09-25T20:23:58Z
dc.date.available2024-09-25T20:23:58Z
dc.date.issued2019
dc.departmentAbant İzzet Baysal Üniversitesien_US
dc.description.abstractAim:This study investigates the effects of two direct factor Xa inhibitors, apixaban and rivaroxaban, and a direct thrombin inhibitor, dabigatran, on human primary chondrocytecultures.Materials and Methods:Monolayer cultured chondrocytes were prepared. Cell cultures were treated with dabigatran, apixaban, and rivaroxaban. Cultures without drug treatments served as the control group. Using an inverted light microscope, the cell surface morphology was examined. Cell viability and the toxicity of drugs were evaluated using a commercial assay kit, and the results were confirmed using two nucleic acid binding dyes, acridine orange and propidium iodide. The expressions of cartilage oligomeric protein, matrix metalloproteinase-7, and matrix metalloproteinase-19 were assessed using the real-time polymerase chain reaction analysis. All the analyses were performed within 21 days. The data obtained were statistically evaluated.Results:The administration of the three drugs changed the cell viability, proliferation, and expressions of cartilage oligomeric protein, matrix metalloproteinase-7, and matrix metalloproteinase-19. The results were statistically significant (P<0.05).Conclusion:Results obtained from in vitro studies may not provide accurate and reliable insight for clinical practices. However, clinicians should know that drugs used for the prevention or treatment of diseases may suppress chondrocyte proliferation and damage the extracellular matrix formation.en_US
dc.identifier.endpage208en_US
dc.identifier.issn2587-0262
dc.identifier.issue3en_US
dc.identifier.startpage201en_US
dc.identifier.trdizinid345934en_US
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/345934
dc.identifier.urihttps://hdl.handle.net/20.500.12491/16258
dc.identifier.volume7en_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.relation.ispartofNamık Kemal Tıp Dergisien_US
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmzYK_20240925en_US
dc.titleA STUDY ON THE EFFECTS OF DIRECT FACTOR XA INHIBITORS AND DIRECT THROMBIN INHIBITORS ON HUMAN PRIMARY CHONDROCYTE CULTURESen_US
dc.typeArticleen_US

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