Investigation of Complexing Properties with Polyethyleneimine of Some Commercial Lipases

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dc.contributor.authorKoç, Eda Öndül
dc.contributor.authorYilmaz, Mert
dc.date.accessioned2024-09-25T20:12:51Z
dc.date.available2024-09-25T20:12:51Z
dc.date.issued2024
dc.departmentAbant İzzet Baysal Üniversitesien_US
dc.description.abstractLipases are enzymes used in various industrial process and are immobilized to increase their applicability as biocatalysts. Ionic polymers such as polyethyleneimine (PEI) make possible the co-precipitation of enzymes. In this study, complexation and aggregation with PEI of enzymes were investigated with commercial enzymes from Novozyme 51032 (Fusarium solani pisi), Palatase 20000 L (Rhizomucor miehei), Lipolase 100 L (Thermomyces lanuginosus), Lipozyme CAL B L (Candida antarctica B) and Amano (Pseudomonas fluorescens) using PEI as a linker and aggregation agent. The highest percentage of PEI-enzyme agregate was obtained for Novozyme 51032, Palatase 20000 L and Lipolase 100 L at the PEI/enzyme ratio of a 1/20-1/80 range. This study documented that Lipozyme CAL B L and (Amano) P. fluorescens enzyme preparations failed to occur precipitates resulting PEI-enzyme aggregates. The some commercial lipase preparations may contain various impurity components that prevent complexation or aggregation with PEI. Complexing with PEI of lipases is based on of basis electrostatic interaction of enzyme with PEI as a cationic polymer resulting in PEI-lipase aggregates.en_US
dc.identifier.doi10.53433/yyufbed.1319182
dc.identifier.endpage199en_US
dc.identifier.issn1300-5413
dc.identifier.issn2667-467X
dc.identifier.issue1en_US
dc.identifier.startpage189en_US
dc.identifier.trdizinid1233249en_US
dc.identifier.urihttps://doi.org/10.53433/yyufbed.1319182
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1233249
dc.identifier.urihttps://hdl.handle.net/20.500.12491/14705
dc.identifier.volume29en_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.relation.ispartofYüzüncü Yıl Üniversitesi Fen Bilimleri Enstitüsü Dergisien_US
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmzYK_20240925en_US
dc.subjectBiocatalysten_US
dc.subjectLipaseen_US
dc.subjectPEI-lipase agregateen_US
dc.subjectPolyethyleneimineen_US
dc.titleInvestigation of Complexing Properties with Polyethyleneimine of Some Commercial Lipasesen_US
dc.typeArticleen_US

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