Influence of the wavelength and intensity of LED lights and cytokinins on the growth rate and the concentration of total cardenolides in Digitalis mariana Boiss. ssp. heywoodii (P. Silva and M. Silva) Hinz cultivated in vitro
Yükleniyor...
Tarih
2022
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Springer
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Digitalis mariana Boiss. ssp. heywoodii is a source of bioactive cardenolides like glucoevatromonoside. The aim was to investigate the in vitro propagation of D. mariana ssp. heywoodii under different cytokinin concentrations and combinations, as well as distinct light intensities and wavelengths, and their effect on plant growth and total cardenolide production. Shoots were inoculated in MS culture medium supplemented with the following 6-benzylaminopurine (BAP) + thidiazuron (TDZ) cytokinin combinations. Shoots were additionally grown under different light intensities (20, 50, 78, 102 and 139 mu mol m(-2) s(-1) LEDs) and wavelength conditions (fluorescent, white, green, blue (B), red (R) LEDs-70%R:30%B, 30%R:70%B, 50%R:50%B). The largest number of shoots was induced at BAP + TDZ concentrations of 0.5 + 0.5; 0.25 + 0.75 and 1.0 + 0.5 mg L-1, with mean values of 8.80, 10.37 and 10.60 shoot explants(-1), respectively. The combination of 0.375 + 0.125 mg L-1 of BAP + TDZ (366 mg plant(-1)) resulted in the highest dry leaf weight. Increased cytokinin concentrations led to a larger number of hyperhydrified shoots and decreased cardenolide production. The absence of cytokinins in the medium resulted in higher contents of total cardenolides (0.310 +/- 0.008%, g/g) in leaves. However, the combination of 0.375 mg L-1 BAP + 0.125 mg L-1 TDZ increased the total cardenolide production to 82.52 +/- 12.52 mg TC plantlet(-1). Light intensity of 139 mu mol m(-2) s(-1) improved plant growth and cardenolide production. With respect to the light spectrum the blue/red wavelength combination encouraged shoot biomass production and elevated total cardenolide contents. Key message Different cytokinin concentrations, light intensities and combinations of blue and red LEDs result in efficient biomass production and high yields of total cardenolides of Digitalis mariana Boiss. ssp. heywoodii.
Açıklama
The authors thanks to National Council for Scientific and Technological Development (CNPq-Conselho Nacional de Desenvolvimento Cientifico e Tecnologico), the Minas Gerais State Research Foundation (FAPEMIG-FundacAo de Pesquisa do Estado de Minas Gerais), and the Coordination for the Improvement of Higher Education Personnel (CAPES-CoordenacAo de Aperfeicoamento de Pessoal de Nivel Superior-Brasil (CAPES-Finance Code 001) for providing the necessary equipment and technical support for the experiments. The authors extend thanks to CAPES and CNPq for granting doctoral (G.M.S.) and productivity scholarships (F.C.B.; J.E.B.P.P.; S.K.V.B.). Mobility also became possible through grants from EU FP7 IRSES grant 295251 (W.K.), CNPq 490057/2011-0 (F.C.B.) and CNPq 406003/2016-5 (S.K.V.B.). Barbara White is acknowledged for linguistic advice.
Anahtar Kelimeler
Propagation in Vitro, Plantaginaceae, Photon Flux Density, Light-Emitting Diode, Spectrophotometry, Mediated Genetic-Transformation
Kaynak
Plant Cell Tissue and Organ Culture
WoS Q Değeri
Q2
Scopus Q Değeri
Q1
Cilt
151
Sayı
1
Künye
da Silva, G. M., Mohamed, A., de Carvalho, A. A., Pinto, J. E. B. P., Braga, F. C., de Pádua, R. M., ... & Bertolucci, S. K. V. (2022). Influence of the wavelength and intensity of LED lights and cytokinins on the growth rate and the concentration of total cardenolides in Digitalis mariana Boiss. ssp. heywoodii (P. Silva and M. Silva) Hinz cultivated in vitro. Plant Cell, Tissue and Organ Culture (PCTOC), 151(1), 93-105.
