In vitro regeneration and analysis of total phenolics in Ocimum basilicum L. (sweet basil)
Küçük Resim Yok
Tarih
2011
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Georg Thieme Verlag Kg
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
An efficient in vitro regeneration system via direct and indirect shoot organogenesis was developed from cotyledonary leaf and hypocotyl explants of Ocimum basilicum L., commonly known as a sweet basil, belonging to the family Lamiaceae. Sweet basil is used in traditional medicine as a culinary herb and a well known source of flavouring properties. Various types and concentrations (ranging from 0.1 to 3.0mg/l) of plant growth regulators in different combinations (TDZ+IAA, BAP+IAA, KIN+IAA, TDZ+NAA) were tested using Murashige and Skoog medium. The highest number of shoots (1.3 shoots per explant) was obtained from hypocotyl explants on medium supplemented with the growth regulators 6-benzylaminopurine (BAP) and indole-3-acetic acid (IAA). For rooting, regenerated shoots were transferred to auxin-containing media. After the rooting stage, healthy regenerants were transferred to pots for acclimitization process, through which almost all of them grew vigorously, attained maturity and produced fertile seeds. We also analysed variations in patterns of total phenolics in the in vitro cultured callus, regenerated plantlets from callus as well as in leaves of ex vitro plants by UV-spectrophotometer. The phenolic contents of regenerated plantlets and leaves of ex vitro plants were found very similar but considerably higher than the callus. However, the greatest difference of phenolics content between callus and regenerated plantlets was observed when they were cultured on media containing combinations of BAP and IAA; callus produced 492,75mg/g dry weight (dw) while regenerated plantlets yielded 1258,81mg/g dw.
Açıklama
59th International Congress and Annual Meeting of the Society-for-Medicinal-Plant-and-Natural-Product-Research -- SEP 04-09, 2011 -- Antalya, TURKEY
Anahtar Kelimeler
Ocimum Basilicum
Kaynak
Planta Medica
WoS Q Değeri
Q2
Scopus Q Değeri
Cilt
77
Sayı
12
