Sand-wounding of shoot and petiole explants enhances transformation efficiency in sugar beet (Beta vulgarisL.) by agrobacterium-mediated transformation

Basit Öğe Kaydı
dc.authorid0000-0003-2379-5363en_US
dc.contributor.authorGürel, Songül
dc.date.accessioned2021-06-23T19:17:09Z
dc.date.available2021-06-23T19:17:09Z
dc.date.issued2020
dc.departmentBAİBÜ, Fen Edebiyat Fakültesi, Biyoloji Bölümüen_US
dc.description.abstractGenetic transformation of recalcitrant species, like sugar beet, has been always a challenging task. In this study, a selectable marker gene (phosphomannose isomerase) and a reporter gene (synthetic green fluorescent protein) were transferred to sugar beet viaAgrobacterium-mediated transformation by using sand-wounded shoot and petiole explants of two different diploid sugar beet genotypes. Considering the overall means of genotypes and explant types, a clear genotypic variation was evident as ELK345 produced a mean of 5.7% transient expression and 1.97% stable transformation efficiency while it was 4.3% and 1.28% for the genotype M1195. When explant types were compared independently from other parameters, it was shown that petiole explants were more productive than shoot explants, with an overall mean of 5.3% versus 4.7% and 1.77% versus 1.48% transient expressions and stable transformation efficiencies, respectively. On the other hand, when a comparison was made without taking into account the genotypes, the effect of wounding treatment on transient expression resulted in about 25% (4.7% vs. 5.9%) and 20% (4.3% vs. 5.1%) increases in petiole and shoot explants, respectively. Such a promoting effect of wounding was even more prominent when the stable transformation efficiencies were compared; petiole explants achieving almost a 60% increase (1.38% vs. 2.16%) while shoot explants doubled (0.98% vs. 1.97%) their efficiencies. The optimized protocol reported here can be employed for further increasing the transformation efficiency in recalcitrant plant species including sugar beet. The advantages of using GFP as a reporter and mannose as a positive selection are also discussed.en_US
dc.identifier.doi10.1007/s12355-020-00893-0
dc.identifier.endpage427
dc.identifier.issn0972-1525
dc.identifier.issue2
dc.identifier.scopus2-s2.0-85091163939en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage415
dc.identifier.urihttps://doi.org/10.1007/s12355-020-00893-0
dc.identifier.urihttps://hdl.handle.net/20.500.12491/5254
dc.identifier.volume23
dc.identifier.wosWOS:000571607500002en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.institutionauthorGürel, Songül
dc.language.isoenen_US
dc.publisherSpringer Indiaen_US
dc.relation.ispartofSugar Techen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectSugar Beeten_US
dc.subjectBeta Vulgaris Len_US
dc.subjectAgrobacteriumen_US
dc.subjectTransformationen_US
dc.subjectWoundingen_US
dc.subjectSanden_US
dc.titleSand-wounding of shoot and petiole explants enhances transformation efficiency in sugar beet (Beta vulgarisL.) by agrobacterium-mediated transformationen_US
dc.typeArticleen_US

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim Yok
İsim:
songul-gurel.pdf
Boyut:
1.33 MB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam metin/ Full text
İndir

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Biyoloji Bölümü Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu