A NOVEL AND SEMIAUTOMATED ASSAY FOR THIOL OXIDATIVE STRESS INDEX: TOSI

Basit Öğe Kaydı
dc.contributor.authorNeselioglu, Salim
dc.contributor.authorŞenat, Almila
dc.contributor.authorOğuz, Esra Firat
dc.contributor.authorAlişik, Murat
dc.contributor.authorErel, Özcan
dc.date.accessioned2024-09-25T20:23:45Z
dc.date.available2024-09-25T20:23:45Z
dc.date.issued2020
dc.departmentAbant İzzet Baysal Üniversitesien_US
dc.description.abstractObjective: The aim of the study is to develop a new andautomated method determining irreversible and reversible thioloxidation products which are formed by the exposure of theplasma to oxidation and to measure the resistance of thiolgroups to oxidation.Material and Methods: The method is based on thioldisulphidehomeostasis measurement of plasma thiol groupsbefore and after exposure to oxidative stress. According to themeasurements, the levels of reversible thiol oxidation products(RTOP), irreversible thiol oxidation products (ITOP), and thioloxidative stress index (TOSI=ITOP/RTOP) were determined.Results: Plasma levels of ITOP were 24.02±12.1 ?mol/L, levelsof RTOP were 26.68±14.96 ?mol/L and TOSI was 0.67±0.60 inthe healthy control group. The level of ITOP of patients withlung and colon cancer were found to be significantly higher thanthe control group, while the level of RTOP was found to besignificantly lower. When the parameter of TOSI which wasused to determine the direction of the balance was examined, itwas observed that the formation of ITOP was more dominant inboth colon and lung cancer patients when compared to thecontrol group.Conclusion: Practical, inexpensive and semi-automaticspectrophotometric RTOP, ITOP and TOSI tests, developed byus for the first time, can be used to investigate many diseasesassociated with oxidative stress.en_US
dc.identifier.doi10.24938/kutfd.674608
dc.identifier.endpage280en_US
dc.identifier.issn2148-9645
dc.identifier.issue2en_US
dc.identifier.startpage271en_US
dc.identifier.trdizinid418921en_US
dc.identifier.urihttps://doi.org/10.24938/kutfd.674608
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/418921
dc.identifier.urihttps://hdl.handle.net/20.500.12491/16101
dc.identifier.volume22en_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.relation.ispartofKırıkkale Üniversitesi Tıp Fakültesi Dergisien_US
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmzYK_20240925en_US
dc.titleA NOVEL AND SEMIAUTOMATED ASSAY FOR THIOL OXIDATIVE STRESS INDEX: TOSIen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

TR-Dizin İndeksli Yayınlar Koleksiyonu