Development of a competent and trouble free dna isolation protocol for downstream genetic analyses in glycine species

Basit Öğe Kaydı
dc.authorid0000-0002-7470-0080
dc.authorid0000-0001-6037-0546
dc.authorid0000-0002-1916-2785
dc.contributor.authorNawaz, Muhammad Amjad
dc.contributor.authorBaloch, Faheem Shehzad
dc.contributor.authorRehman, Hafiz Mamoon
dc.contributor.authorLe, Bao
dc.contributor.authorAkther, Fahima
dc.date.accessioned2021-06-23T18:41:39Z
dc.date.available2021-06-23T18:41:39Z
dc.date.issued2016
dc.departmentBAİBÜ, Ziraat Fakültesi, Tarla Bitkileri Bölümüen_US
dc.description.abstractExtraction of deoxyribose nucleic acid (DNA) from plants is preliminary step in molecular biology. Fast and cost effective genomic DNA isolation from Glycine species for downstream application is a major bottleneck. Here we report a high throughput and trouble free method for genomic DNA extraction from leaf and seeds of Glycine species with high quality and quantity. Protocol reports the optimization by employing different concentrations of CTAB and PVP in extraction buffer. Efficiency of optimized protocol was compared with frequently used DNA extraction methods. Wide adoptability and utility of this protocol was confirmed by DNA extraction from leaves as well as seeds of G. max, G. soja, G. tomentella and G. latifolia. Extracted DNA was successfully subjected to PCR amplification of five microsatellite markers and four putative glycosyltransferase genes. DNA extraction protocol is reproducible, trouble free, rapid and can be adopted for plant molecular biology applications.en_US
dc.identifier.endpage705en_US
dc.identifier.issn2148-127X
dc.identifier.issue8en_US
dc.identifier.startpage700en_US
dc.identifier.trdizinidTR-DizinIDen_US
dc.identifier.urihttps://app.trdizin.gov.tr/makale/TWpBek5UQTRPQT09
dc.identifier.urihttps://hdl.handle.net/20.500.12491/3449
dc.identifier.volume4en_US
dc.indekslendigikaynakTR-Dizinen_US
dc.institutionauthorBaloch, Faheem Shehzad
dc.language.isoenen_US
dc.relation.ispartofTürk Tarım - Gıda Bilim ve Teknoloji dergisien_US
dc.relation.ispartofTurkish Journal of Agriculture - Food Science and Technology (TURJAF)
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCTABen_US
dc.subjectDNA Extractionen_US
dc.subjectGlycine Species
dc.subjectGenetic Analyses
dc.subjectPVP
dc.titleDevelopment of a competent and trouble free dna isolation protocol for downstream genetic analyses in glycine speciesen_US
dc.typeArticleen_US

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim
İsim:
muhammad-amjad-nawaz.pdf
Boyut:
454.72 KB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam metin / Full text
İndir

Koleksiyon

TR-Dizin İndeksli Yayınlar Koleksiyonu
Tarla Bitkileri Bölümü Koleksiyonu