Clonal propagation, antioxidant activity and phenolic profiles of Convolvulus galaticus Rostan ex Choisy
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Dosyalar
Tarih
2018
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Ars Docendi
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Convolvulus galaticus Rostan ex Choisy (grizzle bindweed) is a medicinal plant in the family Convolvulaceae. The first objective of this study was to determine a highly efficient and rapid regeneration system for C. galaticus. Secondly, field-grown and in vitro-grown plants were compared in terms of antioxidant activities and phenolic constituents. C. galaticus leaves and stems were surface sterilized and three different explants (leaf stem and node) were cultured. Regeneration was observed only with node explants. Best shoot proliferation was observed with 0.5 mg/l TDZ and 1.0 mg/l IAA, producing 15 shoots per explant at 84 % frequency. In vitro regenerated plants were also used as donor plants for explant source and best shoot formation was observed with 1.0 mg/l TDZ and 0.5 mg/l IBA with node explant, producing 23.7 shoots per explant at 60 % frequency. Regenerated shoots were transferred to rooting media and 1.0 mg/l IBA was the most effective for rooting. In the second part of this study, methanolic extract of field-grown and in vitro-grown C. galaticus were compared in terms of antioxidant activity and phenolic constituents. Field-grown plant showed higher antioxidant activities and phenolic content than in vitro-grown plant.
Açıklama
Anahtar Kelimeler
Antioxidant, Convolvulus galaticus, In Vitro Culture, LC-MS/MS, Phenolics
Kaynak
Romanian Biotechnological Letters
WoS Q Değeri
Q4
Scopus Q Değeri
Cilt
23
Sayı
3
