Comparison of the effects of operating microscopes with light emitting diodeand halogen light source on the eye: A rabbit study
Yükleniyor...
Dosyalar
Tarih
2021
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Taylor & Francis Ltd
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Purpose To evaluate the potential toxicity of operation microscopes with halogen and light emitting diode (LED) light source on the rabbit eyes. Materials and methods Thirty-two eyes of 16 male New Zealand pigmented rabbits were involved in the study. The rabbits were divided into two groups according to the type of light source applied. Only one eye of each rabbit was exposed to illumination light, unexposed fellow eyes served as the control group. Experimental groups included group 1 exposed to halogen light for 2 h and evaluated 1 day and 1 week after the illumination, group 2 exposed to LED light for two hours and evaluated 1 day and 1 week after the illumination. On the first and seventh days after exposing the light, we evaluated the rabbit corneas using in vivo confocal microscopy (IVCM). At the end of the seventh day, the Hematoxylin-eosin staining and TUNEL staining were performed to investigate the presence of apoptosis in the retina and retina pigment epithelium. Results Early IVCM findings revealed corneal epithelial cell ovalization and indistinct intercellular borders in the halogen light group. We also observed more increase in the keratocyte density index (23.7% vs 14.1%, p = 0.001, respectively) and the Bowman reflectivity index (12.4% vs 4.1%, p = 0.001, respectively) at first day of the light exposure in halogen light group compared to LED light group. However, late IVCM indicated that these findings disappeared one week later. No apoptosis was observed in the corneal and retinal layers in early and late examination groups. Conclusion The present experimental study demonstrated that both halogen and LED lights, which were commonly used for microscopic eye surgery, had no sustained adverse effect on the cornea and retina of the rabbits; however, halogen light had a temporary adverse effect on corneal epithelium and stroma, which resolved within 1 week.
Açıklama
This work was supported by the Projects of Scientific Investigation, Gazi University under Grant [Project number: 01/2015-16].
Anahtar Kelimeler
Confocal Microscopy, Halogen Light, Light Emitting Diode, İnduced Retinal Phototoxicity, Photochemical Damage, Body-Temperature
Kaynak
Cutaneous and Ocular Toxicology
WoS Q Değeri
Q3
Scopus Q Değeri
Q3
Cilt
40
Sayı
4
Künye
Aydın, B., Ozgur, A., Ozdemir, H. B., Uyar Gocun, P., Inan, M. A., Atalay, H. T., ... & Ucgul, A. Y. (2021). Comparison of the effects of operating microscopes with light emitting diode and halogen light source on the eye: a rabbit study. Cutaneous and Ocular Toxicology, 40(4), 319-325.