Performance of rapid test in detection of anti-HCV in frozen sera
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Dosyalar
Tarih
2014
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Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Amaç: Hepatit C, dünya genelinde yaygın enfeksiyöz bir hastalıktır. Anti-HCV’yi saptayan Hepatit C hızlı testinin duyarlılık ve özgüllüğü düşük olabilmekle birlikte kolay uygulanabilen kullanışlı bir testtir. Bu çalışmada, HCV enfeksiyonu hızlı testinin birkaç yıldır dondurulmuş olarak bekletilen anti-HCV pozitif serum örneklerinde hâlâ doğru sonuçlar verip vermediğinin araştırılması amaçlanmıştır. Gereç ve Yöntemler: Kemilüminisans teknolojisini kullanan sistemle (Architect i2000 sr, IL, ABD) anti-HCV pozitif saptanan toplam 100 hastaya ait serumlar -20 °C’de en fazla 3,5 yıllık bir süre saklandı. Serumlar çözdürüldü ve Nanosign hızlı test kiti (Bioland, Güney Kore) kullanılarak anti-HCV için tekrar test edildi. Bulgular: Testin pozitiflik oranı %37 olarak bulundu. Ek olarak, hızlı test, anti-HCV düzeyi 10 S/CO’nun altında olan serumlarda %5,2 gibi çok düşük bir pozitiflik oranı verdi. Bunun tersine, yüksek anti-HCV düzeyi bulunan örneklerde bu oran %71,4 idi. Pozitiflik dereceleri ve anti-HCV düzeyleri arasında anlamlı pozitif korelasyon bulundu (r=0,708, p<0,001). Pozitiflik dereceleri ile dondurulma üzerinden geçen süre arasında herhangi bir korelasyon bulunmadı (r=-0,91, p=0,367). Bunun yanı sıra, dondurulma zaman aralıkları bakımından gruplar arasında anlamlı bir farklılık gözlenmedi (p>0,05). Sonuç: Bu çalışmanın bulguları, örneklerdeki moleküllerin kararsızlığına bağlı olarak HCV hızlı testinin düşük anti-HCV bulunduran dondurulmuş serumlarda güvenilir olmadığını ve örneklerin dondurulmasından sonra geçen sürenin test sonuçlarını değiştirmediğini göstermektedir.
Objective: Hepatitis C is a common infectious disease throughout the world. Rapid test of hepatitis C, detecting anti-HCV, is a useful test that is easy to perform as well as it may have lack of sensitivity and specificity. In the present study, it was aimed to investigate whether the rapid test of HCV infection can still reveal accurate results in anti-HCV-positive sera frozen for a few years. Materials and Methods: Sera of a total of 100 patients that were determined to be positive for anti-HCV using chemiluminescence technology system (Architect i2000sr, IL, USA) were stored at -200 °C for a maximum period of 3.5 years. The sera were thawed and tested again for anti-HCV using the Nanosign rapid test kit (Bioland, Korea) for HCV infection. Results: The positivity rate of the test was 37%. In addition, the rapid test revealed a very low positivity rate as 5.2% in sera with a level of anti-HCV below 10.0 S/CO. In contrast, the positivity rate was 71.4% in samples with high anti-HCV levels. A significant positive correlation was found between positivity levels and anti-HCV levels (r=0.708, p<0.001). No correlation was found between positivity levels and time passed after freezing the samples (r=-0.91, p=0.367). Besides this, no signif- icant difference was observed amongst the groups in terms of the time intervals of freezing (p>0.05). Conclusion: The findings of this study suggest that the rapid test of HCV infection is not reliable in frozen sera with low anti-HCV levels due to the instability of the molecules in the samples, and the time passed after freezing of the sample doesn’t change the results of the tests.
Objective: Hepatitis C is a common infectious disease throughout the world. Rapid test of hepatitis C, detecting anti-HCV, is a useful test that is easy to perform as well as it may have lack of sensitivity and specificity. In the present study, it was aimed to investigate whether the rapid test of HCV infection can still reveal accurate results in anti-HCV-positive sera frozen for a few years. Materials and Methods: Sera of a total of 100 patients that were determined to be positive for anti-HCV using chemiluminescence technology system (Architect i2000sr, IL, USA) were stored at -200 °C for a maximum period of 3.5 years. The sera were thawed and tested again for anti-HCV using the Nanosign rapid test kit (Bioland, Korea) for HCV infection. Results: The positivity rate of the test was 37%. In addition, the rapid test revealed a very low positivity rate as 5.2% in sera with a level of anti-HCV below 10.0 S/CO. In contrast, the positivity rate was 71.4% in samples with high anti-HCV levels. A significant positive correlation was found between positivity levels and anti-HCV levels (r=0.708, p<0.001). No correlation was found between positivity levels and time passed after freezing the samples (r=-0.91, p=0.367). Besides this, no signif- icant difference was observed amongst the groups in terms of the time intervals of freezing (p>0.05). Conclusion: The findings of this study suggest that the rapid test of HCV infection is not reliable in frozen sera with low anti-HCV levels due to the instability of the molecules in the samples, and the time passed after freezing of the sample doesn’t change the results of the tests.
Açıklama
Anahtar Kelimeler
Hızlı Test, Anti-HCV, Dondurulmuş Serumlar, HCV, Rapid Test, Anti-HCV, Frozen Sera, HCV
Kaynak
Viral Hepatitis Journal
WoS Q Değeri
Scopus Q Değeri
Cilt
20
Sayı
1