Genetic diversity of some tomato cultivars and breeding lines commonly used in Pakistani breeding program

dc.authorid0000-0003-1525-8373
dc.authorid0000-0002-7470-0080
dc.contributor.authorShah, Muhammad Azhar
dc.contributor.authorKhan, Azeem Iqbal
dc.contributor.authorAwan, Faisal Saeed
dc.contributor.authorSadaqat, Hafeez Ahmed
dc.contributor.authorBahadur, Sultan
dc.contributor.authorBaloch, Faheem Shehzad
dc.date.accessioned2021-06-23T18:41:38Z
dc.date.available2021-06-23T18:41:38Z
dc.date.issued2015
dc.departmentBAİBÜ, Ziraat Fakültesi, Tarla Bitkileri Bölümüen_US
dc.description.abstractGenetic diversity present in gene pool is an important determination for breeding programs, and characterization is useful of building crop plant collections primarily based on the knowledge of the presence of valuable genes and traits. Developing successful varieties for increasing the future yield and quality of tomato depend mainly on the genetic diversity of parents used in the breeding program. Molecular characterization of 21 tomato genotypes used in in Pakistani breeding program was studied using random amplified polymorphic DNA (RAPD) markers. Total 102 bands were amplified among 21 genotypes using 20 RAPD primers. Overall 73.5% polymorphism was shown as 75 out of 102 loci were polymorphic. High degree of divergence between varieties was indicated by low level of monomorphic bands. The number of PCR products per primer varied from 2-8 with an average of 5.1 bands per primer. Primer GL J-20 and GL C-09 produced maximum number of bands whereas the primers GL A-09 produced the lowest. The polymorphism per RAPD primer ranged from 50% to 100% with an average of 73.5%. The accumulative analysis of amplified products generated by RAPD’s was enough to assess the genetic diversity among the genotypes. The information would be helpful for formulating future breeding and genome mapping programs. This study will also work as an indicator for tomato breeders to evolve varieties with genetic diverse back ground to achieve sustainability in tomato production in the country.en_US
dc.identifier.endpage132en_US
dc.identifier.issn2148-127X
dc.identifier.issue3en_US
dc.identifier.startpage126en_US
dc.identifier.trdizinid188043en_US
dc.identifier.urihttps://app.trdizin.gov.tr/makale/TVRnNE1EUXpNdz09
dc.identifier.urihttps://hdl.handle.net/20.500.12491/3448
dc.identifier.volume3en_US
dc.indekslendigikaynakTR-Dizinen_US
dc.institutionauthorBaloch, Faheem Shehzad
dc.language.isoenen_US
dc.relation.ispartofTürk Tarım - Gıda Bilim ve Teknoloji dergisien_US
dc.relation.ispartofTurkish Journal of Agriculture - Food Science and Technology (TURJAF)
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectGenetic Relationshipen_US
dc.subjectDiversityen_US
dc.subjectDNA Markers
dc.subjectPakistan
dc.subjectTomato
dc.titleGenetic diversity of some tomato cultivars and breeding lines commonly used in Pakistani breeding programen_US
dc.typeArticleen_US

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