Effects of Nebivolol on Skin Flap Survival: A Randomized Experimental Study in Rats
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Dosyalar
Tarih
2008
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
BACKGROUND: Skin flaps are among the basic treatment options in the reconstruction of soft tissue defects. To improve skin flap survival, a variety of methods, including pharmacologic agents, have been investigated. The effectiveness of anticoagulants, antioxidants, anti-inflammatory drugs, and vasodilatory drugs in improving flap survival has been studied. Nebivolol isa new-generation selective beta(1)-adrenoreceptor blocking agent that has vasodilatory, antithrombotic, antioxidative, and anti-inflammatory effects. OBJECTIVE: The aim of this experimental study was to investigate the effects of nebivolol (50 mg/kg/d) on random pattern skin flap survival in rats. METHODS: Male Wistar rats weighing 290 to 310 g were randomly divided into 2 groups-the nebivolol group and the control group. Random patterned, caudally-based, similar to 3 x 10-cm skin flaps were elevated on the back of each rat. In the nebivolol group, nebivolol 50 mg/kg/d (1 mL of a racemic solution of nebivolol) was administered orally 2 days before surgery to reach steady-state drug blood concentrations and was continued for 6 days. In the control group, 1 mL/d of sterile saline solution was orally administered 2 days before surgery and was continued for 6 days. To observe the effects of nebivolol, cutaneous blood flow was examined using a laser Doppler flowmeter before and after Surgery on days 1, 3, 5, and 7, and flap tissue, malondialdehyde (MDA) and glutathione (GSH) concentrations, and Superoxide dismurase (SOD) activity were measured 7 days postsurgery. Flap viability was evaluated 7 days after surgery by measuring necrotic flap area and total flap area. RESULTS: All 20 rats (nebivolol group, n = 10; control group, n = 10) survived throughout the Study period. Mean (SD) MDA concentration was significantly lower in the nebivolol group than in the control group (69-25 [5-82] vs 77.67 [6.87] nmol/g tissue; P = 0.009). GSH concentration was significantly higher in the nebivolol group than in the control group (2.14 [0.15] vs 1.88 [0.22] nmol/mg tissue; P = 0.004). SOD activity was significantly greater in the nebivolol group than in the control group (49.28 [5.49] vs 42.09 [4.95] U/g tissue; P = 0.007). The percentage of the flap that was necrotic was significantly lower in the nebivolol group than in the control group (40.27 [4.08] vs 48.87 [6-35]; P = 0.007). CONCLUSIONS: This small, experimental, in vivo animal Study found that nebivolol was associated with reduced necrotic random pattern skin flap area. Further Studies are needed to clarify these findings. (Curr Ther Res Clin Exp. 2008;69:449458) (c) 2008 Excerpta Medica Inc.
Açıklama
Anahtar Kelimeler
Nebivolol, Skin Flap Survival, Cutaneous Blood Flow
Kaynak
Current Therapeutic Research-Clinical And Experimental
WoS Q Değeri
Q4
Scopus Q Değeri
Q3
Cilt
69
Sayı
5
