Pestisit tayini için nanopartiküllerle duyarlılığı arttırılmış moleküler baskılama temelli yüzey plazmon rezonans sensörlerin hazırlanması
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Dosyalar
Tarih
2018
Dergi Başlığı
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Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Pestisitler yıllardır tarım alanlarında kullanılmaktadır. Ancak bu yaygın kullanım, bilimsel ve endüstriyel topluluklarda pestisitlerin uzun vadeli insan sağlığı riskleri üzerine bilinen toksisitelerinden dolayı endişeyi arttırmıştır. Böylelikle pestisitlerin tayin edilmesi, hem insanlar üzerinde artan toksik etkilerinden hem de kullanımının kontrol altına alınmasından dolayı büyük bir öneme sahiptir. Bu çalışmada, seçici, hassas, hızlı ve gerçek zamanlı klorpirifos tayini için ilk olarak, poli[etilenglikol dimetakrilat-N-metakriloil-(l)-triptofan metil ester poly(EGDMA-MATrp) nanopartikülleri hazırlandı ve zeta sizer ile karakterize edildi. Ardından, klorpirifos baskılı SPR nanosensör hazırlanarak atomik kuvvet mikroskobu (AFM) ve temas açısı ölçümleri ile karakterize edildi. Kalınlık ölçümleri ve AFM görüntüleri, nanopartiküllerden oluşan ince filmlerin hemen hemen tek tabakalı olduğunu gösterdi. Baskılanmış ve baskılanmamış nanosensörlerin klorpirifos tayin duyarlılığı 0.015-2.9 nM aralığında klorpirifos çözeltileri ile araştırıldı. Baskılanmış nanopartiküllerin, baskılanmamışlara göre daha fazla duyarlılık gösterdiği belirlendi. Langmuir adsorpsiyon modeli, bu afinite sistemi için en uygun model olarak belirlendi. Klorpirifos baskılanmış nanopartiküllerin seçiciliğini göstermek için 2.9 nM derişimindeki klorpirifos, diazinon ve parationun yarışmalı adsorpsiyonu araştırıldı. Sonuç olarak, SPR nanosensörlerin hızlı yanıt, kullanım kolaylığı, hassasiyet, seçicilik ve gerçek zamanlı ölçüm alınabilme özelliğinden dolayı pestisit analizleri için alternatif bir yöntem olarak kullanılma potansiyeline sahip olduğu görüldü.
Pesticides have been utilized in agriculture for decades. However, their widespread use has increased concerns due to their known toxicities on long term human health risks in scientific and industrial communities. Thus, the detection of pesticides will have a great prescription due to both improve their toxicity effects over humans and their management. In this study, poly[ethyleneglycol dimethacrylate-N-methacryloyl-(L)-tryptophan methyl ester poly(EGDMA-MATrp) nanoparticles for sensitive, selective, fast and realtime detection chlorpyrifos were firstly prepared and characterized by zeta sizer. Then, a chlorpyrifos-printed SPR nanosensor was prepared and characterized by atomic force microscopy (AFM) and contact angle measurements. The thickness measurements and AFM observations indicated that the nanoparticle thin films were almost monolayer. Chlorpyrifos sensing ability of imprinted and nonimprinted nanosensors were investigated from chlorpyrifos solutions within the range of 0.015-2.9 nM. Imprinted nanoparticles showed more sensitivity to chlorpyrifos than non-imprinted ones. Langmuir adsorption model was determined as the most suitable model for this affinity system. In order to show the selectivity of the chlorpyrifos imprinted nanoparticles, competitive adsorption of chlorpyrifos, diazinon and parathion at 2.9 nM concentration was investigated. As a result of, SPR nanosensors have a potential to be used as an alternative method for pesticides analysis due to fast response, easy-to-use properties, precision, selective and real-time measurement capability.
Pesticides have been utilized in agriculture for decades. However, their widespread use has increased concerns due to their known toxicities on long term human health risks in scientific and industrial communities. Thus, the detection of pesticides will have a great prescription due to both improve their toxicity effects over humans and their management. In this study, poly[ethyleneglycol dimethacrylate-N-methacryloyl-(L)-tryptophan methyl ester poly(EGDMA-MATrp) nanoparticles for sensitive, selective, fast and realtime detection chlorpyrifos were firstly prepared and characterized by zeta sizer. Then, a chlorpyrifos-printed SPR nanosensor was prepared and characterized by atomic force microscopy (AFM) and contact angle measurements. The thickness measurements and AFM observations indicated that the nanoparticle thin films were almost monolayer. Chlorpyrifos sensing ability of imprinted and nonimprinted nanosensors were investigated from chlorpyrifos solutions within the range of 0.015-2.9 nM. Imprinted nanoparticles showed more sensitivity to chlorpyrifos than non-imprinted ones. Langmuir adsorption model was determined as the most suitable model for this affinity system. In order to show the selectivity of the chlorpyrifos imprinted nanoparticles, competitive adsorption of chlorpyrifos, diazinon and parathion at 2.9 nM concentration was investigated. As a result of, SPR nanosensors have a potential to be used as an alternative method for pesticides analysis due to fast response, easy-to-use properties, precision, selective and real-time measurement capability.
Açıklama
Anahtar Kelimeler
Moleküler Baskılama, Nanopartikül, Pestisit, Yüzey Plazmon Rezonans Sensör, Molecular Imprinting, Nanoparticles, Pesticide, Surface Plasmon Resonance Sensor
Kaynak
Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi
WoS Q Değeri
Scopus Q Değeri
Cilt
8
Sayı
3
