Deney Hayvanları Uygulama ve Araştırma Merkezi
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Öğe Effects of Nigella sativa seeds and certain species of fungi extracts on number and activation of dural mast cells in rats(Akademiai Kiado Rt, 2017) Kılınç, Erkan; Dağıstan, Yaşar; Kotan, Batuhan; Çetinkaya, AyhanIn this study, we aimed to investigate the effects of Nigella sativa seeds and certain species of fungi extracts on the number and degranulation states of dural mast cells in rats. Rats were fed ad libitum with normal tap water or tap water with extract of N. sativa seed, Ramaria condensata, Lactarius salmonicolor, Lactarius piperatus, and Tricholoma terreum for 3 days. Mast cells in dura mater were counted and evaluated in terms of granulation and degranulation states. Compound 48/80, a mast cell degranulating agent, and T. terreum significantly increased the percent of degranulated mast cells in dura mater, respectively (p < 0.01 and p < 0.05). Moreover, T. terreum causes a significant increase in the total number of mast cells (p < 0.05). N. sativa significantly inhibited mast cell degranulation induced by both the compound 48/80 and T. terreum (p < 0.05), and significantly decreased the mast cell numbers increased by T. terreum (p < 0.05). Our results suggested that T. terreum following ingestion can contribute to headaches like migraine via dural mast cell degranulation and N. sativa may be able to exert analgesic and anti-inflammatory effects by stabilizing dural mast cells. However, investigation is needed to determine the ingredients of N. sativa that may be responsible for these beneficial effects.Öğe Ameliorating effect of hawthorn (Crataegus oxyacantha) and physical exercise on acute penicillin induced seizures in gerbils(African Networks Ethnomedicines, 2016) Çakır, Serkan; Orallar, Hayriye; Çetinkaya, Ayhan; Kayacan, Yıldırım; Önal, Ali Can; Yıldırım, Arzu; Benek, Selim; Özkan, Murat; Okur, NezihBackground: The aim of the present study was to assess the effect of Hawthorn (Crataegus oxyacantha) and physical activity. We studied its effect on penicillin induced epilepsy. in gerbils. Materal and Methods: Epilepsy was induced by administration of peniciline G (500 IU, ip). The gerbils were divided randomly in four groups (6 animals per each group) and studied as described below: 1) Control group 2) Exercise group (30 min/each day for 8 weeks) (Eg) 3) Extract group, 50mg/kg/day/animal in 1 ml saline, 3 h prior to exercise (Exe) 4) Exercise+ Extract + (Exe+ Ex). The severity of epilepsy was observed and recorded. Results: The means of latencies (Mean +/- SE) were 236 +/- 45, 369 +/- 36, 386 +/- 58 and 433 +/- 37 ms in groups of control, Exe, Ex, and Exe+ Ex respectively. The mean spike latency significantly (P= 0,033 F= 3,560) decreased in Exe, Ex and Exe+ Ex when compared control. Although spike frequency significantly (P< 0.05) diminished in groups of Exe and Ex, no significant decrease was observed in control and Exe+ Ex. Similar trend was seen for amplitude values. Spike amplitude values were determined to be significantly (P< 0.05) lower than those of control and Exe+ Ex. Conclusion: Crataegus oxyacantha extract has shown positive affect to ameliorate on some seizure parameters in this study. However, further more advanced physiologic and neurochemical studies are required to determine the mechanisms involved.Öğe Protective effects of rosmarinic add on doxorubicin-induced testicular damage(Karger, 2014) Üyetürk, Ümmügül; Üyetürk, Uğur; Fırat, Tülin; Çetinkaya, Ayhan; Tekçe, Buket Kın; Çakır, SerkanBackground: We investigated the protective effects of rosmarinic acid (RA) on testicular damage induced by doxorubicin (DXR) in rats. Methods: In total, 21 rats were divided into 3 groups: the control group that received no treatment, the DXR group that received intraperitoneal (i.p.) DXR on day 7 and the DXR + RA group that received intra-gastric RA for 10 days with i.p. DXR on day 7. The rats were sacrificed on day 11 for histological and biochemical analyses. To assess oxidative damage, glutathione peroxidase (GPx) and malondialdehyde (MDA) levels were measured. Results: The median modified Johnsen score of the DXR + RA group was higher than that of the DXR group (p = 0.002). The rats with the narrowest seminiferous tubules were in the DXR group (0.17 +/- 0.03), and the difference between the DXR + RA and DXR groups was statistically significant (p = 0.002). The number of apoptotic cells in the DXR group was significantly higher than that in the control group, and there were significantly fewer apoptotic cells in the DXR + RA group than in the DXR group (p = 0.002). The MDA level was lowest in the control group and highest in the DXR group, and the level observed in the DXR + RA group significantly lower than that in the DXR group (p = 0.002). The GPx level was highest in the control group, with the level observed in the DXR + RA group significantly higher than that in the DXR group (p = 0.002). The testosterone level was lowest in the DXR group and highest in the control group, and that observed in the DXR + RA group was significantly higher than that in the DXR group (p = 0.018). Conclusions: RA can correct DXR-induced testicular damage through its antioxidant properties. However, the mechanism underlying the effects of RA requires further investigation, and long-term and comparative human studies are also needed. (C) 2014 S. Karger AG, BaselÖğe Intraperitoneal nigella sativa for prevention of postoperative intra-abdominal adhesions in rats(Informa Healthcare, 2014) Karataş, Ahmet; Özlü, Tülay; Özyalvaçlı, Gülzade; Tosun, Mehmet; Çetinkaya, Ayhan; Dönmez, Melahat Emine; Türker, ArzuObjective: To evaluate intraperitoneal administration of Nigella sativa (NS) to prevent postoperative intraperitoneal adhesion (PPA) after surgical manipulation of rat uterine horn. Materials and Methods: Two forms of NS were used in the study (Volatile oil (NSVO) and the ethanolic extract (NSEE)). A total of 50 rats were randomly assigned to the sham group (n = 10), control group (n = 10), NSVO group (n = 10), NSEE group (n = 10), and the Seprafilm group(n = 10). After 14 days, rats were sacrificed. Adhesions were examined macroscopically, and degree of adhesions was scored. A part of horn was excised, and superoxide dismutase (SOD) and glutathione peroxidase activities as well as malondialdehyde levels were evaluated, and histological score was calculated. Results: Total microscopic score of the NSEE groupwas significantly lower than the control group (p = .001) and was marginally significantly lower than the seprafilm group (p = .005). Collagen formation score was higher in the seprafilm group compared to the sham and NSEE groups (p < 0.001, p = .003, respectively). Apoptotic cells were lower in the NSEE group compared to the control group (p=. 003) and also lower in the NSEE and NSVO groups compared to the seprafilm group (p = .001, p < .001, respectively). Only SOD activity was higher in the NSVO and seprafilm groups compared to the control group (p < .001). Conclusion: NSEE form seems to have a possible effect in the prevention of PPAs. This may occur by its effect in decreasing collagen formation and by decreasing apoptosis in the injured tissues. NSVO form seems to induce SOD. Therefore, combined use of NSVO with seprafilm may increase the adhesion preventive effect of seprafilm.Öğe Alleviation of kidney damage induced by unilateral ureter obstruction in rats by rhodiola rosea(Mary Ann Liebert, Inc, 2013) Üyetürk, Uğur; Terzi, E. Hakan; Kemahlı, Eray; Gücük, Adnan; Tosun, Mehmet; Çetinkaya, AyhanPurpose: To evaluate the efficacy of Rhodiola rosea extract in terms of alleviating the renal damage induced by unilateral ureter obstruction (UUO) in rats. Material and Methods: Thirty Wistar albino male rats were divided into five groups: (I) Control, (II) UUO 7 days, (III) UUO 7 days+extract,(IV) UUO 14 days, and (V) UUO 14 days+extract. Seven or 14 days after the initiation of the experimental procedure, the left kidneys of rats in all five groups were removed for histological examination, and their blood was drawn for biochemical measurements. Result: Median malondialdehyde (MDA) and glutathione peroxidase (GPx) levels were, respectively, 39.4 (5.04) nmol/mL and 25.8 (8.01) nmol/minute/mL in group I, 77.9 (12.38) nmol/mL and 5.8 (1.95) nmol/minute/mL in group II, 48.7 (12.1) nmol/mL and 9.1 (2.3) nmol/minute/mL in group III, 58.5 (23.83) nmol/mL and 8.4 (2.1) nmol/minute/mL in group IV, and 44.8 (4.97) nmol/mL and 13.8 (3.73) nmol/minute/mL in group V. There was a statistically significant difference among the groups in terms of MDA and GPx levels (p<0.05 for both). The median numbers of apoptotic cells were 1 (1), 8 (2.25), 3 (1.25), 23.5 (9), and 7 (I) in groups I, II, III, IV, and V, respectively. There was a statistically siginificant difference among the groups in terms of apoptotic cell number (p<0.05). Conclusion:R. rosea extract was shown to alleviate the renal damage induced by UUO through its antioxidant effects. The mechanism by which R. rosea extract causes these effects merits further investigation.Öğe Hydrated sodium calcium aluminosilicate for reduction of aflatoxin in quails (Coturnix coturnix japonica)(M H Schaper Gmbh Co Kg, 2007) Şehu, Adnan; Ergün, Lütfi; Çakır, Serkan; Ergün, Emel; Cantekin, ZaferThe purpose of the present study was to evaluate the toxic effects of aflatoxin (AF) on growth performance and various processing parameters of quails and to determine the preventive efficacy of hydrated sodium calcium aluminosilicate (HSCAS). One hundred and eighty 1-d-old quails of both sexes were randomly divided into 4 experimental groups with 5 replicates and 45 birds following weighing.The experimental design consisted of four dietary treatments: 1) control with 0 mg AF/kg of diet and 0 % HSCAS; 2) 0.5 % HSCAS; 3) 2.5 mg AF/kg of diet; 4) 2.5 mg AF/kg of diet plus 0.5 % HSCAS.The chicks were housed in electrically heated battery cages and exposed to light for 24 h from hatching to 3 weeks of age. Quails consumed the diets and water od libitum. Body weight (BW) was significantly (p < 0.001) increased by addition of HSCAS to AF diet. The lowest BW gains in groups received AF alone was observed at all periods. The reduction in BW gain caused by 2.5 mg AF/kg of diet was significantly (p < 0.001) diminished by the addition of 0.5 % HSCAS to the diet. The addition of HSCAS to the AF diet significantly (p < 0.001) protected against decrease of feed intake at all periods with exception of the first period. None of the treatments altered significantly the feed conversion ratio (FCR).The relative weights of the liver, kidney and spleen were increased in the chickens consuming the AF alone diet. However, light microscopic examination demonstrated the addition of HSCAS to quail feed to partially decrease fat deposition caused by the toxin, and besides, electron microscopic examination of indicated a reorganization in the encloplasmic reticulum and increase in the number of ribosomes and polisomes. Furthermore, the decrease in the antibody titre induced by Newcastle vaccine, due to aflatoxins, was relatively prevented. No significant differences were observed for serum total protein, total cholesterol and glucose levels. The results of indicate that HSCAS is effective in preventing the deleterious effects of AFÖğe Abant İzzet Baysal Üniversitesi Eğitim Araştırma Hastanesine başvuran hastalarda 6 yıllık toxoplasma gondii seropozitifliğinin araştırılması(2018) Türkoğlu, Şule Aydın; Karabörk, Şeyda; Çakmak, Mücahit; Orallar, Hayriye; Yaman, Kerem; Ayaz, ErolAmaç: Toxoplasma gondii, beyin, kalp, göz tutulumuna neden olabilen hücre içi parazit enfeksiyon etkenidir. Abant İzzet Baysal Üniversitesi Eğitim ve Araştırma Hastanesine Ocak 2010-Aralık 2016 yılları arasında Toxoplasmosis şüphesi ile başvuran Hastalarda T.gondii IgG ve IgM antikorlarının prevalansının saptanması amaçlanmıştır. Yöntem: Abant İzzet Baysal Üniversitesi Eğitim ve Araştırma Hastanesine başvuran kişilere ait 14262 serum örneğinde ELİSA yöntemi ile belirlenen anti-T.gondii IgM ve IgG antikorları ve IgG avidite testi retrospektif olarak araştırılmıştır. Bulgular: 13607’ü kadın (%95,4), 560’ü erkek (%3,9), 95’i ise bebek (%0,7) toplam 14262 bireyden T.gondii antikorlarının belirlemesi için istem yapılmıştır. IgG bakılan 4079 olguda %78’i negatif, %21’i pozitif, %0,8 grayzone belirlenmiştir. IgM bakılan 13671 olgunun %98’i negatif, %1,2’si pozitif, %0,5 grayzone belirlenmiştir. Avidite testine göre 135 olgunun IgG avidite test sonuçlarında 45 (%33) olgu düşük, 20 (%15) olgu sınır değer, 70 (%52) hastada yüksek avidite belirlenmiştir. IgG seropozitifliği erkek ve kadınlarda IgM pozitifliğine göre yüksek bulunmuştur. İstem yapılan olguların çoğunluğunun gebelik takibi nedeniyle Kadın Doğum kliniğinden (n=12588) (%88,3) istendiği, Enfeksiyon Hastalıkları (n=540) (%3,8) ve Nöroloji (n=478) (%3,4) kliniklerinden T.gondii antikorlarının tespiti için istem yapıldığı görülmüştür. Sonuç: T.gondii seropozitifliğinin ilimizde ihmal edilemeyecek ölçüde yaygın olduğu görülmüştür. Riskli grup içerisindeki olguların bu parazit açısından değerlendirilmeye alınması ve farkındalığın oluşturulması gerektiği sonucuna varılmıştır. Son yıllarda özellikle Nöroloji kliniğinde bu farkındalığın oluştuğu görülmüştür.
