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    Effects of plant growth regulators, different culture media and strength MS on production of volatile fraction composition in shoot cultures of Ocimwn basilicum
    (Elsevier Science Bv, 2018) Monfort, Lucila Elizabeth Fragoso; Bertolucci, Suzan Kelly Vilela; Lima, Andreisa Fabri; de Carvalho, Alexandre Alves; Mohammed, Aliyu
    The influence of different culture media, strength MS media and plant growth regulators (PGR) was assessed through the growth and production of the volatile fraction composition in Ocimwn basilicum (basil). The treatments were: the use of different culture media (MS, B5 and WPM), strength MS media (2MS, MS, 1/2MS and 1/4MS) and different PGR combinations or only IAA, NAA, IBA, BAP and TDZ. The leaf number, nodal segment, shoot number, lenght, shoot dry weight, root number, root size, root dry weight, as well as the volatile fraction analysis through Head-Space - GC/MS were assessed at the 40th culture day. The present results suggest that media type, MS concentration and the growth regulator types have qualitative and quantitatively influenced the growth and volatile composition. A higher amount of methyleugenol in the media 2MS and 1/4MS, and higher content of linalool and 1,8-cineole in MS and WPM were observed. The PGR improved biomass production and induced quantitative modifications in the production of major volatile constituents in Ocimwn basilicum plant lets, although the same major compounds were produced in all treatments. There was a tendency to estragole synthesis in the presence of auxin combined with cytokinin. There was a tendency to linalool synthesis in the presence of auxin isolates.
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    Explant type and natural ventilation systems influence growth and content of carvacrol and thymol of Lippia gracilis Schauer
    (Springer, 2019) Santos Lazzarini, Luiz Eduardo; Vilela Bertolucci, Suzan Kelly; de Carvalho, Alexandre Alves; Santiago, Alexsandro Carvalho; Pacheco, Fernanda Ventorim; Yücesan, Bahtiyar Buhara; Brasil Pereira Pinto, Jose Eduardo
    The objective of this study was to evaluate the effects of different natural ventilation systems and explant types on the growth and volatile compound content of Lippia gracilis cultured in vitro. The treatments consisted of four membrane systems (without membrane, with one, two, and four porous membranes) and two explant types (nodal segments with and without a pair of leaves). The evaluation of growth, photosynthetic pigments and chemical analysis of the volatile fraction were performed at 35days of cultivation in half strength MS basal medium. Natural ventilation systems significantly influenced the in vitro growth and volatile fraction of L. gracilis. Explants with a pair of leaves obtained the best experimental responses. The natural ventilation system with four membranes provided the best growth parameters and leaf area response of L. gracilis explants with leaves. The photosynthetic pigments decreased with an increase in the number of porous membranes in the culture flask. Variations in the number, content, and profile of volatile compounds under the influence of natural ventilation systems were observed. Major constituents such as -cymene, -terpinene, thymol, carvacrol, and E-caryophyllene, regardless of experimental conditions, were identified. The highest carvacrol and thymol contents were observed in plantlets grown in culture flasks with four porous membranes. To maximize the content of carvacrol and thymol from the in vitro culture of L. gracilis, explants with a pair of leaves and four porous membranes in culture flasks are recommended for use.