Yazar "Varol, Muhammed Ismail" seçeneğine göre listele

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    Anti-proliferative effects of salmon calcitonin on SH-SY5Y neuroblastoma in vitro
    (2023) Varol, Muhammed Ismail; Karabörk, Şeyda; Çetinkaya, Ayhan
    Aim: We aimed to examine the potential cytotoxic effect of salmon calcitonin, which is one of the components that regulates mineral metabolism and prevents the increase in the amount of calcium, on SH-SY5Y cells, a neuroblastoma cell line.Methods: SH-SY5Y cells were cultured in DMEM medium in the presence of 37°C and 5% CO2in conventional culture flasks. MTT assay was applied to investigate the effect of calcitonin individually on SH-SY5Y cells by treatment different concentrations for 24 h and performed. Results: In cells cultured with salmon calcitonin applied at different concentrations (0.1, 1, 3.125, 6,25, 12.5, 25, 50 and 100 nM/ml), anti-proliferation was statistically significant at concentrations of 50 and 100 nM/ml compared to the control group. It showed that 50 nM/ml and 100 nM/ml had the highest cytotoxic effect on SH-SY5Y for 24 hConclusions: Considering the proliferation curve of SH-SY5Y, the results show that salmon calcitonin treatment potentiated the proliferative activities by inhibiting cell viability in SH-SY5Y cells at concentrations of 50 and 100 nM/ml. Further studies exploring salmon calcitonin’s protective effects may prove successful and maybe it is a promising agent for cancer treatmen
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    PHYLOGENETIC RELATIONSHIPS OF SOME PARDOSA (LYCOSIDAE) SPECIES
    (Parlar Scientific Publications (P S P), 2021) Akpinar, Adile; Yuncu, Aynur; Arslan, Derya; Lucas, James Stuart; Gurer, Turkan; Varol, Muhammed Ismail
    In this study, 3 Pardosa species ( Pardosa proxima (C. L. Koch, 1847), P. tatarica (Thorell, 1875), P. italica Tongiorgi, 1966) that have similar genital morphologies were analyzed by using the mitochondrial cytochrome oxidase 1 (COI) gene region. Haplotypes belonging to P. proxima and P. tatarica populations were determined. Pardosa proxima populations were exposed to low intraspecific mutation and exhibited a balancing selection. P. tatarica populations had a high mutation rate and an expanding population. The best evolutionary model that includes all samples were identified and a phylogenetic tree containing relationships between haplotypes was created. Similarities between intra and inter species were calculated. Morphologically similar species were found to be molecularly close as well. This findings show that P. proxima populations are not affected by environmental factors and their morphological characters are preserved. In P. tatarica populations, it was observed that the rate of morphological variation was high and affected by environmental factors.