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    The effect of pretreating seedlings with bap on direct shoot regeneration from petiole explants of sugar beet (Beta vulgaris L.)
    (2003) Gürel, Ekrem; Topal, Emel; Songül, Gürel
    A 100% seed sterility and 75.6% seed germination rate were achieved when seeds of three sugar beet (Beta vulgaris L.) breeding lines were surface sterilized with a combination of 0.5% Domestos and 5% PPM™. As an in planta treatment with BAP, the sterilized seeds were germinated on MS basal medium containing 1, 3 or 5 mg/l BAP for five weeks and then petiole explants were excised from the seedlings and cultured on two different regeneration media; RGL medium containing 0.5 mg/l BAP and 0.1 mg/l NAA, and RGH medium containing 1.0 mg/l BAP and 0.5 mg/l NAA. Petiole explants taken from seedlings pretreated with 1 mg/l BAP produced significantly more shoots (0.97 shoot per explant) than those taken from seedlings pretreated with 3 or 5 mg/l BAP (0.68 and 0.59 shoot per explant, respectively). The RGL medium containing lower BAP and NAA was significantly more effective in direct shoot production than the RGH medium containing higher BAP and NAA, 1 shoot per explant compared to 0.48, respectively. 21.1% of the regenerated shoots developed roots on medium containing 3 mg/l NAA whereas only 4.1% of the shoots rooted on medium containing 1 mg/l NAA. Genotypic variation was significantly evident for both shoot regeneration and rooting. © 2003 Taylor and Francis Group, LLC.
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    The effect of pretreating seedlings with TDZ on direct shoot regeneration from petiole explants of sugar beet (Beta vulgaris L.)
    (University of Malaya, 2003) Songül, Gürel; Topal, Emel; Gürel, Ekrem
    A 100% seed sterility and 75.6% seed germination rate were achieved when seeds of three sugar beet (Beta vulgaris L.) breeding lines were surface sterilized with a combination of 0.5% Domestos and 5% PPM™. The procedures we describe here are more successful than the previously published procedures as a high rate of sterility was achieved without reducing the germinability of sugar beet seeds. As an in planta treatment with TDZ (thidiazuron), the sterilized seeds were germinated on MS basal medium containing 1, 3 or 5 mh/L TDZ for five weeks and then petiole explants were excised from the seedlings and cultured on two different regeneration media; RGL medium containing 0.5 mg/L BAP and 0.1 mg/L NAA, and RGH medium containing 1.0 mg/L BAP and 0.5 mg/L NAA. Petiole explants taken from seedlings pretreated with 1 mg/L TDZ produced significantly more shoots (1.67 shoots per explant) than those taken from seedlings pretreated with 3 or 5 mg/L TDZ (1.1 6 and 0.92 shoots per explant, respectively). The RGL medium containing lower BAP and NAA was significantly more effective in direct shoot production than the RGH medium containing higher BAP and NAA, 1.54 shoots per explant compared to 0.96, respectively. Such an increase in regeneration capacity of the explants due to the TDZ pretreatment is clearly significant for sugar beet, which is regarded as a recalcitrant species. 42.6% of the regenerated shoots developed roots on medium containing 3 mg/L NAA whereas only 19.3% of the shoots rooted on medium containing 1 mg/L NAA. Genotypic variation was significandy evident for both shoot regeneration and rooting.
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    The Effect of the composition of germination medium on in vitro regeneration of sugar beet (Beta vulgaris L. )
    (Bolu Abant İzzet Baysal Üniversitesi, 2002) Topal, Emel; Gürel, Ekrem
    ÖZET ŞEKER PANCARI {BETA VULGARIS L.) ISLAH HATLARINDA ÇİMLENDİRME ORTAMI İÇERİĞİNİN REJENERASYON ÜZERİNE ETKİSİ TOPAL, Emel Yüksek Lisans Tezi, Biyoloji Bölümü Tez Danışmanı: Doç. Dr. Ekrem GÜREL Ağustos 2002, 77 sayfa Üç farklı ıslah hattına ait şeker pancarı tohumları sterilize edildikten sonra TDZ ve BAP'nin çeşitli kombinasyonlarını içeren MS ortamında çimlendirilmişlerdir. Kontrol amaçlı olarak MS basal ortamı kullanılmıştır. Bu şekilde elde edilen steril fidelere ait petiyol eksplantları 1.0 mg/1 BAP + 0.3 mg/1 NAA {RG-I ortamı) veya 0.5 mg/1 BAP + 0.1 mg/1 NAA {RG-II ortamı) içeren iki farklı rejenerasyon ortamına aktarılmış ve yaklaşık 4-5 gün sonra direkt rejenerantlar gözlenmiştir. Elde edilen direkt rejenerantlar 1.0 mg/1 NAA veya 3.0 mg/1 NAA içeren iki farlı köklendirme ortamında kültüre alınmışlardır. Kontrol ortamında (MS basal) çimlendirilerek elde edilen eksplantlardan hiçbirinde doğrudan rejenerasyon gözlemlenmezken diğer çimlendirme ortamlarından elde edilen viiexplantlarda direkt rejenerasyon oranları hatlar ve ortamlar arasında değişiklik göstermiştir. Rejenerasyon ortamlarından ise 0.5 mg/1 BAP + 0.1 mg/1 NAA ortamı istisnasız bütün hatlarda diğer rejenerasyon ortamına göre daha başarılı sonuçlar vermiştir. Köklendirme ortamlarından; 3.0 mg/1 NAA ortamı, 1.0 mg/1 NAA ortamına nazaran (tüm hatlar için) daha başarılı olmuştur. Ayrıca TDZ çimlendirme ortamından elde edilen eksplantlar BAP çimlendirme ortamından elde edilen eksplantlara göre daha fazla oranda kök gelişimi göstermişlerdir. Anahtar kelimeler: Beta vulgaris L., şeker pancarı, sterilizasyon, in vitro kültür, çimlednirme ortamı, direkt sürgün rejenerasyonu. viii