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    Selection and Preparation of Explants for the Clonal Propagation of Horticultural Plants in Plant Factory Systems
    (Springer Singapore, 2022) Tigrel, Ahmet; Arslan, Merve; Arıcı, Beyza; Yücesan, Buhara
    Explant preference is a key factor for efficient and sustainable plant propagation under in vitro conditions. Plant genotype and structure must be well observed and identified for the best explant which may differ in the axillary bud breakings using terminal buds on stems located above ground or specialized/underground stems such as bulbs scales, base plates of corms, and the shoot tips of suckers. Since plant factory systems are aimed at uniform and cost-effective propagation systems, determination of explant type and culture conditions are the most critical factors for the establishment of shoot multiplication rate. In this chapter, several horticulture plants including house plants (Monstera, Philodendron, Begonia, etc.), and fruit trees (Aronia, banana, walnut, etc.) used in commercial-scale production in plant factories were investigated for the understanding of the nature of explants as per culture conditions. This phenomenon is also highly correlated with effective surface sterilization. Since plant factories rely on an automation system for particular crops, replenishment of starting material in each cloning cycle prevents the emergence of undesirable traits due to the somaclonal variations. This study reports a comparative and in situ analysis of explant choice for the scalable vitro-plant productions. © The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Nature Singapore Pte Ltd. 2022.