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Öğe Alleviation of kidney damage induced by unilateral ureter obstruction in rats by rhodiola rosea(Mary Ann Liebert, Inc, 2013) Üyetürk, Uğur; Terzi, E. Hakan; Kemahlı, Eray; Gücük, Adnan; Tosun, Mehmet; Çetinkaya, AyhanPurpose: To evaluate the efficacy of Rhodiola rosea extract in terms of alleviating the renal damage induced by unilateral ureter obstruction (UUO) in rats. Material and Methods: Thirty Wistar albino male rats were divided into five groups: (I) Control, (II) UUO 7 days, (III) UUO 7 days+extract,(IV) UUO 14 days, and (V) UUO 14 days+extract. Seven or 14 days after the initiation of the experimental procedure, the left kidneys of rats in all five groups were removed for histological examination, and their blood was drawn for biochemical measurements. Result: Median malondialdehyde (MDA) and glutathione peroxidase (GPx) levels were, respectively, 39.4 (5.04) nmol/mL and 25.8 (8.01) nmol/minute/mL in group I, 77.9 (12.38) nmol/mL and 5.8 (1.95) nmol/minute/mL in group II, 48.7 (12.1) nmol/mL and 9.1 (2.3) nmol/minute/mL in group III, 58.5 (23.83) nmol/mL and 8.4 (2.1) nmol/minute/mL in group IV, and 44.8 (4.97) nmol/mL and 13.8 (3.73) nmol/minute/mL in group V. There was a statistically significant difference among the groups in terms of MDA and GPx levels (p<0.05 for both). The median numbers of apoptotic cells were 1 (1), 8 (2.25), 3 (1.25), 23.5 (9), and 7 (I) in groups I, II, III, IV, and V, respectively. There was a statistically siginificant difference among the groups in terms of apoptotic cell number (p<0.05). Conclusion:R. rosea extract was shown to alleviate the renal damage induced by UUO through its antioxidant effects. The mechanism by which R. rosea extract causes these effects merits further investigation.Öğe Deneysel karaciğer hasarının akciğer dokusuna etkisi ve mast hücrelerinin rolü(Türkiye Klinikleri Tıp Bilimleri Dergisi, 2013) Fırat, Tülin; Ulaş, Nilüfer; Terzi, E. Hakan; Töre, Fatma; Kükner, AyselAmaç: Deneysel karaciğer hasarına bağlı oluşan akciğer dokusu ve mast hücre sayısındaki değişikliklerin ışık mikroskobik olarak incelenmesi. Gereç ve Yöntemler: Bu çalışmada yetişkin Wistar albino ratlar kullanıldı. Ratlar rastgele, her grupta 6 hayvan olmak üzere 3 gruba ayrıldı. Kontrol grubu, CCL4 grubu ve ligasyon grubu. CCL4 grubunda, CCl4 (1 mL/kg vücut ağırlığı, intraperitoneal), haftada 2 kez toplam 8 hafta uygulandı. Ligasyon grubu, ortak safra kanalı bağlanarak 3 hafta bekletildi. Karaciğer ve akciğer dokularından alınan örnekler hematoksilen-eozin ile boyandı. Akciğer kesitleri toluidin mavisi ile boyanarak parankim ve plevral mast hücreleri sayıldı. Bulgular: Kanal ligasyonu ve CCl4 uygulanan grupların akciğer dokularında interalveolar septum kalınlaşması, kapiller konjesyon, ödem, artmış inflamatuar hücreler ve degranüle mast hücresi tespit edildi. Ligasyon grubunda ise akciğer dokularında bu değişiklikler daha belirgindi. Parankimal ve plevral mast hücre sayısı ligasyon ve CCl4 grubunda kontrole göre anlamlı olarak artmıştı. Sonuç: Hepatopulmoner sendrom olarak tanımlanan karaciğer hasarının neden olduğu akciğer hasarına, artmış mast hücrelerinin aracılık edebileceği tespit edilmiştir.Öğe Prevention of torsion-induced testicular injury by rhodiola rosea(Elsevier Science Inc, 2013) Üyetürk, Uğur; Terzi, E. Hakan; Gücük, Adnan; Kemahlı, Eray; Öztürk, Hayrettin; Tosun, MehmetOBJECTIVE To evaluate the efficacy of Rhodiola rosea (R. rose) extract in terms of preventing tissue injury induced by testicular torsion and subsequent ischemia/reperfusion (I/R). METHODS Twenty-one Wistar albino male rats were divided into 3 groups: group 1 = control group, group 2 = I/R group, and group 3 = I/R + extract group. After 2 hours of ischemia and 4 hours of reperfusion, testes were removed and evaluated histologically by hematoxylin and eosin staining. Apoptosis in spermatogonial cells of seminiferous tubules was determined by transferase biotin-2'-deoxyuridine, 5'-triphosphate nick end labeling (TUNEL). To assess oxidative damage, serum malondialdehyde (MDA) and glutathione (GSH) levels were measured. RESULTS Median MDA and GSH levels were, respectively, 12 +/- 3 pmol/mL and 24.8 +/- 3.8 mu M in group 1, 38 +/- 11 pmol/mL and 10.3 +/- 1.7 mu M in group 2, and 19 +/- 5 pmol/mL and 17.6 +/- 1.3 mu M in group 3 (P <.001 and P <.001, respectively). Median MDA levels, apoptotic cell density, and histopathologic scoring were significantly lower in groups 1 and 3 compared to group 2 (P <.017 for all). Median GSH levels were higher in groups 1 and 3 compared to group 2 (P <.017). CONCLUSION R. rosea extract was shown to have partially preventive effects on testicular injury induced by torsion in this rat model. The mechanism by which R. rosea extract cause these effects merits further investigation. UROLOGY 82: 254.e1-254.e6, 2013. (C) 2013 Elsevier Inc.Öğe The preventive effect of low molecular weight heparin on CCL4-induced necrosis and apoptosis in rat liver(Elsevier Science Bv, 2010) Kükner, Aysel; Töre, Fatma; Fırat, Tülin; Terzi, E. Hakan; Öner, HakanBackground and Aims: Regardless of its etiology, liver injury results in liver failure, cirrhosis, portal hypertension, or hepatocellular carcinoma. Heparin having anti-inflammatory and anti-fibrotic properties may have therapeutic effect on liver injury. The present study investigated the effect of low molecular weight heparin (enoxaparin) on CCL4-induced hepatic necrosis and apoptosis in rats.Öğe The preventive effect of low molecular weight heparin on CCL4-induced necrosis and apoptosis in rat liver(Elsevier Espana, 2010) Kükner, Aysel; Töre, Fatma; Fırat, Tülin; Terzi, E. Hakan; Öner, Hakan; Balaban, Hatice YaseminIntroduction Heparin having anti inflammatory and anti fibrotic properties may have therapeutic effect on liver injury The present study investigated the effect of low molecular weight heparin (Enoxaparin) on carbon tetrachloride (CCl4) induced hepatic necrosis and apoptosis in rats Material and methods Thirty male rats were divided into 5 groups Group I Control, Group II Olive oil dissolved CCl4 at dose of 1 mL/kg, ip, twice per week, Group III CCl4 and Enoxaparin at dose of 180 IU/kg, sc, daily, Group IV Enoxaparin, Group V Olive oil at dose of 1 mL, ip, twice per week The liver histology at the forth week was examined by haematoxylin eosin, Masson's trichrome, Toluidine blue and Periodic acid schiff stains Proliferative and apoptotic activities were assessed semi quantitatively by proliferating cell nuclear antigen (PCNA) and cas pase 3 immune staining and TUNEL method Semi quantitative values formulated by the equation HSCORE = Sigma P-i (1+1) including both distribution and intensity of staining Additionally, mdogen and a smooth muscle actin were labeled by immunohistochemistry Results CCl4 group had marked hepatocelluar necrosis around the vena centralis and increased inflammatory cells and mast cells Hepatocytes showed deposition of lipid droplets, decrease in glycogen, apoptosis, and picnotic or enlarged nuclei Enoxaparin reduced necrosis, apoptosis, and number of mast cells but had no effect on lipid droplets in hepatocytes HSCORE's of caspase 3 and PCNA were also significantly decreased by administration Conclusion Enoxaparin have beneficial effects against necrosis as well as apoptosis at the early stage of CCL4 induced liver injury
