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Öğe Configuring pulsed electric fields to treat seeds: an innovative method of seed disinfection(Ista-Int Seed Testing Assoc, 2017) Evrendilek, Gülsün Akdemir; Tanasov, IgorThis study presents the design and testing of a bench-scale pulsed electric field (PEF) system to process different seeds to reduce the total number of mesophilic aerobic bacteria (TMAB) and fungi (TF). The PEF system was configured to treat seeds at 50 and 200 Hz frequency with 12 kV cm(-1) electric fields strength. PEF treatment was effective in reducing TMAB and TF in the seeds with reduction ranging from 0.22 +/- 0.06 log cfu mL(-1) for cabbage to 2.81 +/- 0.69 log cfu mL(-1) for lettuce for TMAB and from 0.49 +/- 0.10 log cfu mL(-1) for garden rocket to 2.85 +/- 0.8 log cfu mL(-1) for wheat for TF. No significant difference was observed between the control and PEF groups of wheat seeds in terms of germination. It was concluded that the PEF treatment was a promising alternative to reduce initial TMAB and TF values of seeds without an adverse affect on seed germination.Öğe Design and effectiveness of pulsed electric fields towards seed disinfection(Wiley, 2019) Evrendilek, Gülsün Akdemir; Karataş, Berna; Uzuner, Sibel; Tanasov, IgorBACKGROUNDSeeds harbor different microorganisms on their surfaces that degrade seed quality, thus causing an economic loss. Even though different approaches are available for the disinfection of seed surfaces, there is a need to develop environmentally friendly and sustainable technologies. A bench-scale pulsed electric field (PEF) unit was designed to inactivate microflora of eight seeds after which the resultant vigor of the treated seeds was determined. RESULTSSignificant reductions were obtained in endogenous natural and inoculated pathogenic (Alternaria brassica and Xanthomonas campestris pv. campestris, Drechslera graminea and Fusarium graminearum) microflora of seeds. The survival ratios of total aerobic mesophilic bacteria and of total mold and yeast decreased significantly for winter wheat and barley, parsley, onion, lettuce, tomato, and garden rocket with the PEF treatments of 240 and 960J. A significant increase in germination ratio was observed for winter wheat and barley, lettuce, and tomato with 960J. Germination energy increased for parsley with 240J and for winter wheat and barley, lettuce, tomato, and garden rocket with 960J. A better root development and seedling were found for winter barley. CONCLUSIONPEFs are a viable option to both disinfect seed surfaces and improve seed vigor. (c) 2019 Society of Chemical Industry