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    The measurement of alpha amanitin levels using hplc method in Amanita phalloides from Düzce province
    (Duzce University Medical School, 2012) Kaya, Ertu?rul; Karahan, Selim; Hanci, Mustafa; Yaykaşli, Kürşat O?uz; Saritaş, Ayhan; Bayram, Recep; Yilmaz, Ismail
    Aim: The aim of this study is to measure the level of alpha amanitin toxin using HPLC method from Amanita phalloides mushroom collected in the province of Düzce in 2010. Method: One of the mushrooms as a whole body. The other one was extracted after seperated into parts. The measurement was done by HPLC using 303 nm UV wavelength and 250x4,6 mm C18 5 ?m particle included column. Ammonium acetate+methanol+acetonitrile (80+10+10, v/v/v) was used as a mobile phase, and the flow rate was set 1 ml/min. The results were given as a toxin quantity in 1 g dry mushroom. Results: The amount of alpha amanitin was measured as 4,806 mg (±0,033) in the whole body, 3,522 mg (±0,024) in the cap, 5,318 mg (±0,056) in the lamellar, 0,903 mg (±0,004) in the ring, 2,577 mg (±0,037) in the stipe, 0,698 mg (±0,008) in the volva. Conclusion: The level of alpha amanitin in Amanita phalloides from Duzce Province is differ from different countries. Higher and lower levels of toxin than our data obtained investigations are present in the literature. The reason of this differences might be several factors like extraction methods, analysis methods and environmental conditions. © 2012 Düzce Medical Journal.
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    Production of high purity beta amanitin
    (Duzce University Medical School, 2012) Kaya, Ertu?rul; Yaykaşli, Kürşat O?uz; Karahan, Selim; Bayram, Recep; Saritaş, Ayhan; Yaykaşli, Emine
    Objective: Beta amanitin has been used in experiment and has been purified only around 90% purity using existing methods. In this study, it has been aimed to describe the method in order to produce high-purity beta amanitin using preparative HPLC. Methods: Amanita phalloides mushrooms hve been collected, extracted and purified 2 times using preparative HPLC. Validation of the toxin has been performed by comparison of retention time at HPLC and ultraviolet spectrum. Results: Beta amanitin was obtained with 91% (±2.36) purity after first purification process. Beta amanitin was obtained with 99,2% (±0.38) purity after second purification process. It seemed that purified toxin and standard were given maximum absorbance at 303 nm and minumum absorbans at 263 nm, and the structure of the spectrums for both was similar. Conclusion: Beta amanitin with >%99 purity can be produced by this method. © 2012 Düzce Medical Journal.