Yazar "Radek, Renate" seçeneğine göre listele

Listeleniyor 1 - 5 / 5
  • Küçük Resim
    Öğe
    Characteristic light and electron microscopic features of adelina melolonthae, a coccidian pathogen of the european cockchafer, melolontha melolontha (coleoptera/scarabaeidae)
    (SPRINGER INTERNATIONAL PUBLISHING AG, 2021) Yaman, Mustafa; Radek, Renate
    Purpose The aim of this study was to investigate and document the characteristic electron microscopic features of the oocyst of Adelina melolonthae, a coccidian pathogen of the European cockchafer, Melolontha melolontha (Coleoptera: Scarabaeidae). Methods Larvae of M. melolontha were collected at Ordu, Turkey. Each larva was dissected in insect Ringer solution. Adeleid oocysts from the tissue that is suspected to Contain coccidian infections were examined under a light microscope (Zeiss), a scanning electron microscope (FEI Quanta 200) and transmission electron Microscope (Philips EM 208). Results Spherical to ellipsoidal oocysts measure ca. 24-44.5 mu m (mean 35.6 mu m) in diameter (n = 50) and include up to twelve sporocysts. The oocyst wall has double layers; the outer layer measured 400-500 nm in thickness and the inner one 10-25 nm. Sporocysts including two sporozoites are rounded ca. 11-12.5 mu m (mean 11.7 mu m) in diameter. The sporocyst wall consisted of only one 70-80-nm-thick layer. The sporozoites are ellipsoidal and measured 9-11 mu m length and 3-4 mu m in width. Sporocysts include residual bodies. Conclusion In the present paper, the morphology and ultrastructure of the oocyst of A. melolonthae is documented for the first time and compared with other Adelina and coccidian species infecting insects. The results in this study confirm his identification and justify the classification as a separate species A. melolonthae, which differs from other Adelina species.
  • Küçük Resim
    Öğe
    First record of the entomopathogenic protist, Mattesia dispora (Neogregarinorida: Lipotrophidae) of the Mediterranean flour moth, Ephestia kuehniella Zeller (Lepidoptera: Pyralidae) in Turkey
    (Springer, 2019) Yaman, Mustafa; Acar, Kabire Funda; Radek, Renate
    The present study is the first record of a natural neogregarine infection of Ephestia kuehniella Zeller (Lepidoptera: Pyralidae) in Turkey. The infection occurs in the fat body and hemolymph of the larvae. Typical fresh navicular oocysts of the pathogen were 12.1-16.2 mu m in length and 5.9-8.5 in width (n = 50). Oocysts stained with Giemsa stain measured 11.7-14.2 mu m in length and 5.9-8.5 mu m in width. Polar plugs were recognizable using light and electron microscopy. The oocyst wall is quite thick, measuring 460 to 560 nm. Each oocyst contains 8 sporozoites. The described neogregarine has typical characteristics of members of the genus Mattesia (Family Lipotrophidaeorder Neogregarinorida) and shows similarities with Mattesia dispora in morphological and ultrastructural characteristics. The occurrence of the pathogen in different life stages of E. kuehniella was also studied. Total infection was observed as 66.98% in the investigated samples. The pathogen caused different infection levels in the larvae, pupae, and adults of the E. kuehniella, 57.06, 85, and 3.17%, respectively.
  • Küçük Resim
    Öğe
    Mattesia cf. geminata, an ant-pathogenic neogregarine (Apicomplexa: Lipotrophidae) in two Temnothorax species (Hymenoptera: Formicidae)
    (Springer, 2023) Yaman, Mustafa; Kıran, Kadri; Radek, Renate
    An ant-pathogenic neogregarine in Temnothorax affinis and T. parvulus (Hymenoptera: Formicidae) is described based on morphological and ultrastructural characteristics. The pathogen infects the hypodermis of the ants. The infection was mainly synchronous so that only gametocysts and oocysts could be observed simultaneously in the host body. Gametogamy resulted in the formation of two oocysts within a gametocyst. The lemon-shaped oocysts measured 11-13 mu m in length and 8-10 mu m in width. The surface of the oocysts is not smooth but contains many buds. A ring-shaped line containing rosary-arrayed buds line up in the equatorial plane of the oocyst. These specific characteristics were observed for the first time in neogregarine oocysts from ants. Polar plugs were recognizable clearly by light and electron microscopy. The oocyst wall was quite thick, measuring 775 to 1000 nm. Each oocyst contained eight sporozoites. The neogregarines in the two Temnothorax species show many similarities such as the size and shape of the oocysts, a relatively fragile gametocyst membrane, host affinity, and tissue preference. We identified these neogregarines as Mattesia cf. geminata, which is here recorded from natural ant populations in the Old World for the first time. All neogregarine pathogens infecting ants in nature so far have been recorded from the New World. We present the two ant species, Temnothorax affinis and T. parvulus, as new natural hosts for M. cf. geminata. Furthermore, the morphological and ultrastructural characteristics of the oocyst of M. cf. geminata are documented by scanning and transmission electron microscopy for the first time.
  • Küçük Resim
    Öğe
    Morphological, ultrastructural, and molecular identification of a new microsporidian pathogen isolated from Crepidodera Aurata (Coleoptera, Chrysomelidae)
    (2019) Yaman, Mustafa; Algı, Gönül; Radek, Renate
    A new microsporidian pathogen isolated from Crepidodera aurata was identified based on morphological and ultrastructural characteristics, coupled with a molecular phylogenetic analysis. The spores of the microsporidian pathogen were slightly curved in shape, and measured 2.44–3.55 µm in length and 1.25–1.55 µm in width (n = 50). Its ultrastructure is characteristic of monokaryotic groups. All lifecycle stages of the pathogen, including meronts, sporonts, sporoblasts, and mature spores, are monokaryotic. The spore has 6–8 windings of the polar filament. Morphological and ultrastructural characteristics of the lifecycle stages place it within the family Unikaryonidae. However, the phylogenetic tree constructed on the 16S rRNA gene sequence analysis indicates that the pathogen is closely related to the Nosema/Vairimorpha clade of microsporidia. Therefore, we have classified the microsporidian of C. aurata in the tentative group Microsporidium in order to avoid creating an unnecessary or incorrect new genus/species.
  • Küçük Resim
    Öğe
    A new microsporidian pathogen, Vairimorpha gastrophysae sp. nov., isolated from Gastrophysa viridula (Coleoptera: Chrysomelidae)
    (Elseiver GMBH, 2022) Yaman, Mustafa; Aydın, Çiçek; Linde, Andreas; Radek, Renate
    Gastrophysa viridula DeGeer 1775, the green dock leaf beetle, belongs to a group of beneficial insects, which can be used as a classical biological control agent against sorrels (Rumex sp., Polygonaceae). Therefore, any infection by pathogenic organisms in this beetle is undesirable. In the present study, a new microsporidian pathogen isolated from G. viridula was identified based on morphological and ultrastructural characteristics, supported with a molecular phylogenetic analysis. Light and transmission electron microscopy studies indicated that the microsporidium was polymorphic throughout its life cycle. Sporulation stages were not all in direct contact with the host-cell cytoplasm. The fresh single diplokaryotic spores of the secondary sporulation cycle had a long narrow morphology, measuring about 5 x 2.1 mm (n = 50). Octospores produced in the secondary sporulation cycle were also observed. Morphological and ultrastructural characteristics of the life cycle stages place it within the genus Vairimorpha. The phylogenetic tree constructed on the 16S rRNA gene sequence analysis supports the morphological and ultrastructural characteristics and indicates that the pathogen is closely related to the Vairimorpha clade of microsporidia. The pathogen is named Vairimorpha gastrophysae sp. nov. (c) 2022 Elsevier GmbH. All rights reserved.