Yazar "Neselioglu, Salim" seçeneğine göre listele

Listeleniyor 1 - 2 / 2
  • Küçük Resim Yok
    Öğe
    Dynamic thiol-disulfide homeostasis as an oxidative stress marker in ankylosing and undifferentiated
    (Tubitak Scientific & Technological Research Council Turkey, 2023) Ozturk, Cemaleddin; Erten, Sukran; Alisik, Murat; Dogan, Ismail; Ustuner, Guniz Yanik; Neselioglu, Salim; Erel, Ozcan
    Background/aim: Seronegative spondyloarthropathies (SpA) are a group of chronic diseases characterized by axial inflammation, oligoarthritis, and enthesitis. Oxidative stress may contribute to a wide range of rheumatologic diseases, including SpA. This prospective case-control study was designed to compare thiol-disulfide levels as a marker of oxidative stress between SpA patients and healthy controls.Materials and methods: A total of 144 patients diagnosed with undifferentiated spondyloarthropathy (USpA, n = 97) or ankylosing spondylitis (AS, n = 47) were included along with 80 healthy controls. Serum native thiol (NT), total thiol (TT), and disulfide (D) levels were measured using the fully automated Erel method. The ratios NT/TT, D/TT, and D/NT were calculated. Thiol-disulfide levels were compared between the SpA groups and the healthy controls.Results: The NT and NT/TT ratios were found to be significantly lower in the SpA group (p < 0.001). The disulfide, D/NT, and D/TT ratios were found to be significantly higher in the SpA group (p < 0.001). In pairwise comparisons between the SpA subgroups, the NT and TT levels were lower in the USpA group than in the AS group (p = 0.021), but serum disulfide levels were higher in the USpA group than in the AS group (p = 0.004). Among the patients with SpA, the group taking antitumor necrosis factor (anti-TNF) had lower TT measurements compared to the group taking conventional disease modifying antirheumatic drugs (DMARD) (p = 0.039).Conclusion: The thiol-disulfide balance is disturbed in favor of disulfide in SpA patients compared to healthy volunteers. Native and total thiol measurements correlate with acute phase reactants and might be used to monitor disease activity. Anti-TNF therapy might control the oxidative degenerative process better than the conventional DMARD in SpA patients.
  • Küçük Resim Yok
    Öğe
    A NOVEL AND SEMIAUTOMATED ASSAY FOR THIOL OXIDATIVE STRESS INDEX: TOSI
    (2020) Neselioglu, Salim; Şenat, Almila; Oğuz, Esra Firat; Alişik, Murat; Erel, Özcan
    Objective: The aim of the study is to develop a new andautomated method determining irreversible and reversible thioloxidation products which are formed by the exposure of theplasma to oxidation and to measure the resistance of thiolgroups to oxidation.Material and Methods: The method is based on thioldisulphidehomeostasis measurement of plasma thiol groupsbefore and after exposure to oxidative stress. According to themeasurements, the levels of reversible thiol oxidation products(RTOP), irreversible thiol oxidation products (ITOP), and thioloxidative stress index (TOSI=ITOP/RTOP) were determined.Results: Plasma levels of ITOP were 24.02±12.1 ?mol/L, levelsof RTOP were 26.68±14.96 ?mol/L and TOSI was 0.67±0.60 inthe healthy control group. The level of ITOP of patients withlung and colon cancer were found to be significantly higher thanthe control group, while the level of RTOP was found to besignificantly lower. When the parameter of TOSI which wasused to determine the direction of the balance was examined, itwas observed that the formation of ITOP was more dominant inboth colon and lung cancer patients when compared to thecontrol group.Conclusion: Practical, inexpensive and semi-automaticspectrophotometric RTOP, ITOP and TOSI tests, developed byus for the first time, can be used to investigate many diseasesassociated with oxidative stress.