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Öğe Characterization and selection of alfalfa cultivars based on agronomic and quality characteristics information from continental climatic conditions of Turkey(Parlar Scientific Publications (P S P), 2020) Karaköy, Tolga; Nadeem, Muhammad Azhar; Ton, Aybegun; Çilesiz, Yeter; Anlarsal, Adem Emin; Hatipoğlu, RüştüAlfalfa is one of the most important crops mainly grown for forage and hay production in the world. Present study was aimed to investigate the green herbage and hay yield as well as some quality parameters such as acid detergent fiber (ADF), neutral detergent fiber (NDF), digestible dry matter (DDM), crude protein, dry matter contents, organic matter, crude oil and raw fiber of 9 different alfalfa cultivars. This study was conducted during two consecutive years (2015 and 2016) at two locations in Turkey (Sivas -Center and Sivas-Altinoluk). The mean values of ADF, NDF, DDM, crude protein (CP), green forage yield and dry forage yield were 43.73%, 52.69%, 50.70%,19.71%, 59163 kg ha(-1) and 13866 kg ha(-1) respectively. Dry forage yield reflected a positive and highly significant correlation with plant height, number of tillers and fresh forage yield. The varieties called Delta and Azzura performed very well for both locations during the whole period of study and also expressed a great level of winter resistance. Kayseri is a local cultivar in Turkey and it is revealed to be poor in terms of all the studied traits. According to the results of the study, it was concluded that the cultivars, Delta and Azzura, could be successfully grown in the continental climate regions of Turkey, and they could be also used in the breeding program of alfalfa to develop alfalfa varieties with high winter resistance and high yield and quality of forage.Öğe Characterization of genetic diversity in Turkish common bean gene pool using phenotypic and whole-genome DArTseq-generated silicoDArT marker information(Public Library Science, 2018) Nadeem, Muhammad Azhar; Habyarimana, Ephrem; Çiftçi, Vahdettin; Nawaz, Muhammad Amjad; Karaköy, TolgaTurkey presents a great diversity of common bean landraces in farmers' fields. We collected 183 common bean accessions from 19 different Turkish geographic regions and 5 scarlet runner bean accessions to investigate their genetic diversity and population structure using phenotypic information (growth habit, and seed weight, flower color, bracteole shape and size, pod shape and leaf shape and color), geographic provenance and 12,557 silicoDArT markers. A total of 24.14% markers were found novel. For the entire population (188 accessions), the expected heterozygosity was 0.078 and overall gene diversity, Fst and Fis were 0.14, 0.55 and 1, respectively. Using marker information, model-based structure, principal coordinate analysis (PCoA) and unweighted pair-group method with arithmetic means (UPGMA) algorithms clustered the 188 accessions into two main populations A (predominant) and B, and 5 unclassified genotypes, representing 3 meaningful heterotic groups for breeding purposes. Phenotypic information clearly distinguished these populations; population A and B, respectively, were bigger (>40g/100 seeds) and smaller (<40g/100 seeds) seed-sized. The unclassified population was pure and only contained climbing genotypes with 100 seed weight 2-3 times greater than populations A and B. Clustering was mainly based on A: seed weight, B: growth habit, C: geographical provinces and D: flower color. Mean kinship was generally low, but population B was more diverse than population A. Overall, a useful level of gene and genotypic diversity was observed in this work and can be used by the scientific community in breeding efforts to develop superior common bean strains.Öğe Common bean as a potential crop for future food security: An overview of past, current and future contributions in genomics, transcriptomics, transgenics and proteomics(Taylor & Francis, 2021) Nadeem, Muhammad Azhar; Yeken, Mehmet Zahit; Shahid, Muhammad Qasim; Habyarimana, Ephrem; Yılmaz, Hilal; Alsaleh, Ahmad; Hatipoğlu, Rüştü; Çilesiz, Yeter; Khawar, Khalid Mahmood; Ludidi, Ndiko; Ercişli, Sezai; Aasim, Muhammad; Karaköy, Tolga; Baloch, Faheem ShahzadCommon bean is an important legume crop having high quality protein, micronutrients, vitamins and antioxidants, which makes it a "grain of hope" for poor communities. Hence, a good number of breeding activities have been performed on the improvement of various key traits for years. However, recent advancements in molecular markers, sequencing technologies and the completion of the common bean genome sequence have opened numerous opportunities for fine mapping and gene characterization. The availability of these tools together with investigations of quantitative trait loci (QTL) and candidate genes for key traits such as morpho-agronomic, iron and zinc contents, cooking and quality traits, antioxidant activity, biotic and abiotic stresses pave the way to the development of new strategies for common bean genetic improvement. As a food source, it can contribute to the reduction of food scarcity worldwide in the coming years. Therefore, it is very important to take synergic efforts to integrate common bean genetic and genomic resources in breeding activities to ensure food security and contribute significantly to improved livelihoods in developing countries. Moreover, Kompetitive allele specific PCR (KASP) and CRISPR-Cas9 should be used to develop climate resilience common bean varieties. Here, we provide an overview of the evolution of common bean research by highlighting the past and recent advances in genomics, transgenics, transcriptomics and proteomics and also critically discuss the future prospects for further genetic improvement and better expansion of this crop.Öğe Contribution of landraces in wheat breeding(Springer International Publishing, 2021) Nadeem, Muhammad Azhar; Yeken, Mehmet Zahit; Tekin, Mehmet; Mustafa, Zemran; Hatipoğlu, Rüştü; Aktaş, Husnu; Alsaleh, AhmadAgricultural production system is extremely vulnerable to climate change, and this change will heavily affect the grain yields, thereby threating the food security worldwide. People from developing countries are at greatest risk of experiencing food insecurity, and today, millions of people are going to bed hungry. Wheat is serving as a staple food for millions of people around the world. Development of high-yielding wheat varieties during the Green Revolution is considered an important event in agricultural history. However, these plant breeding activities also resulted in genetic erosion in wheat. Moreover, it is also believed that after domestication process, selection process also resulted in the loss of genetic diversity of wheat. Therefore, commercial wheat cultivars are prone to various biotic and abiotic stresses. To combat with climate changes and to serve enough quantity of food with quality, there is a need to harness wheat landraces. Landraces are considered as repository of gene pool that enhance the biodiversity and maintain and stabilize the ecosystem in a sustainable way to make it functional. Wheat landraces are traditional crop populations developed by the farmers through natural and human selection under their years of cultivations and have adaptation to local environment and management practices. Wheat landraces have more genetic diversity compared to their cultivated ones, and breeding community has utilized their potential in development of climate-resilient wheat cultivars. Here, we are exploring the role of landraces in wheat breeding and hoping that provided information will catch the attention of breeding community to collect, conserve, and perform breeding activities using wheat landraces. © The Author(s), under exclusive license to Springer Nature Switzerland AG 2021. All rights reserved.Öğe Determination of Turkish common bean germplasm for morpho-agronomic and mineral variations for breeding perspectives in Turkey(Kahramanmaras Sutcu Imam Univ Rektorlugu, 2019) Yeken, Mehmet Zahit; Nadeem, Muhammad Azhar; Karaköy, Tolga; Baloch, Faheem Shehzad; Çiftci, VahdettinTurkey is lavished with hundreds of common bean landraces. The present study was aimed to investigate the agronomic and mineral variations in 80 common bean landraces collected from 11 different provinces of Turkey. Genotypic variation expressed as a range for some traits like days to maturity (90-141 days), plant height (25.25-361.50 cm), 1000 seeds weight (140-633 g), Iron (66.48-128.05 mg kg(-1)), and Zinc (20.56-42.01 mg kg(-1)). Positive and highly significant correlation of Magnesium with Iron and Zinc was observed and analytic results derived from the first 3 eigenvectors suggested that days to pod setting, Zinc, and 1000 seeds weight were main variation contributing traits. Among the provinces, landraces from Tunceli performed well for agronomic traits and Malatya provinces landraces were found enrich for mineral traits. Landraces E-26 and S-19 reflected higher Fe and Zn contents, and higher yield, respectively. Cluster analysis divided the studied germplasm on the basis of plant height and geographic. Information provided herein can be helpful for the development of candidate varieties having higher yield with greater mineral contents.Öğe DNA fingerprinting and genetic diversity analysis of world quinoa germplasm using iPBS-retrotransposon marker system(Tubitak Scientific & Technical Research Council Turkey, 2020) Barut, Muzaffer; Nadeem, Muhammad Azhar; Karaköy, Tolga; Baloch, Faheem ShehzadQuinoa is an important staple food crop for millions of impoverished rural inhabitants of the Andean region. Quinoa is considered a good source of protein,vitamins, minerals, and antioxidants. This study aimed to investigate the genetic diversity and population structure of world quinoa gennplasm originating from 8 countries through the iPBS-retrotransposon marker system. Molecular characterization was performed using the 11 most polymorphic primers. A total of 235 bands were recorded, of which 66.8% were polymorphic. Mean polymorphism information content (PIC) was 0.410. Various diversity indices including mean effective number of alleles (1.269), mean Shannon's information index (0.160) and gene diversity (0.247) revealed the existence of sufficient amount of genetic diversity in studied germplasm. Bolivia-17 and Mexico-1 were found to be genetically distinct accessions and can be suggested as candidate parents for future breeding activities. Various diversity indices were also calculated among germplasm collection counries and the results clearly showed the existence of higher genetic diversity in Bolivian and Peruvian accessions. The model-based structure, neighbor-joining, and principal coordinate analysis (PCoA) grouped quinoa germplasm according to their collection country. Analysis of molecular variance (AMOVA) revealed that most of the variations (69%) in world quinoa germplasm are due to differences within populations. Findings of this study can be used for deeper understanding of the genetic relationship and in the determination of appropriate breeding and conservation strategies for quinoa.Öğe DNA molecular markers in plant breeding: current status and recent advancements in genomic selection and genome editing(Taylor & Francis Ltd, 2018) Nadeem, Muhammad Azhar; Nawaz, Muhammad Amjad; Shahid, Muhammad Qasim; Doğan, Yıldız; Cömertpay, Gönül; Yıldız, Mehtap; Hatipoğlu, RüştüWith the development of molecular marker technology in the 1980s, the fate of plant breeding has changed. Different types of molecular markers have been developed and advancement in sequencing technologies has geared crop improvement. To explore the knowledge about molecular markers, several reviews have been published in the last three decades; however, all these reviews were meant for researchers with advanced knowledge of molecular genetics. This review is intended to be a synopsis of recent developments in molecular markers and their applications in plant breeding and is devoted to early researchers with a little or no knowledge of molecular markers. The progress made in molecular plant breeding, genetics, genomic selection and genome editing has contributed to a more comprehensive understanding of molecular markers and provided deeper insights into the diversity available for crops and greatly complemented breeding stratagems. Genotyping-by-sequencing and association mapping based on next-generation sequencing technologies have facilitated the identification of novel genetic markers for complex and unstructured populations. Altogether, the history, the types of markers, their application in plant sciences and breeding, and some recent advancements in genomic selection and genome editing are discussed.Öğe Effect of biochar and PGPR on the growth and nutrients content of einkorn wheat (Triticum monococcum L.) and post-harvest soil properties(MDPI, 2021) Çığ, Fatih; Sönmez, Ferit; Nadeem, Muhammad Azhar; Sabagh, Ayman ElLimited availability of nutrients to crops is a major agricultural concern. Deteriorated soil health and poor fertility status decrease the bioavailability of essential nutrients to the plants. Consequently, organic soil amendment biochar is gaining attention due to its potential benefits. Rhizobacterial inoculation, are also documented as an effective technology for mobilization of immobile nutrients in soil. However, limited literature is available on combined use of rhizobacteria and biochar. Therefore, this study was carried out to examine the changes in the nutrient content of einkorn wheat and the change in some soil properties during the application of plant growth-promoting rhizobacteria (PGPR) with biochar. Four doses of biochar (0, 2.5, 5, and 10%) were applied with and without PGPR in the study. Biochar increased the growth criteria such as plant fresh weight (PFW), plant dry weight (PDW), root fresh weight (RFW), root dry weight (RDW), number of tillers, germination rate (GR) and potassium (K), calcium (Ca), sodium (Na), iron (Fe), copper (Cu), zinc (Zn), manganese (Mn), and nickel (Ni) elements. While PGPR application increased soil pH, dry and fresh weight of root, R/S, K, Ca, Mg, Fe, and Ni contents, and it caused a decrease in PH, PFW, tillers, GR, P, Cu, and Zn values. Combined biochar applications and PGPR had a significant effect on the pH, RFW, R/S, P, Na, and Cu. In conclusion, the combination of biochar and PGPR applications has shown a positive effect in terms of soil properties, plant growth, and element contents of einkorn wheat.Öğe Evaluation of yield and yield components of common vetch (vicia sativa l.) genotypes grown in different locations of Turkey by gge biplot analysis(Corvinus Univ Budapest, 2019) Aydemir, Serap Kızıl; Karaköy, Tolga; Kökten, Kağan; Nadeem, Muhammad AzharThis study was carried out under the ecological conditions of three locations in Turkey (Bilecik, Sivas and Bingol) during 2016 aiming to investigate the variations in yield and yield components of common vetch and to evaluate in different environmental conditions the best performing genotypes using the GGE Biplot analysis. 5 lines and 8 commercial cultivars were used as plant material. GGE biplot analysis concluded that plant height, forage yield, biological yield and straw yield were included in component 1. Dicle genotype was found superior in terms of forage yield and dry matter yield, while Kralkizi genotype reflected its potential for biological and straw yield. Findings of this study are suggesting that the common vetch, plant height, forage yield, biologic yield, straw yield are important traits and can be used as selection criteria for the evaluation of best performing common vetch genotypes.Öğe Exploring Genetic Diversity and Population Structure of Turkish Black Sea Region Maize (Zea mays L.) Germplasm using SSR Markers(2022) Baran, Nurettin; Nadeem, Muhammad Azhar; Yilmaz, Abdurrahim; Andirman, Mehtap; Kurt, Fırat; Temiz, M. Gültekin; Baloch, Faheem ShehzadPlant landraces are heterogeneous plant populations that have adapted to local environmental conditions and serve as a source of genetic variations that can be used for the breeding perspectives. Studies regarding genetic diversity assessment are very important as they provide an opportunity for the scientific community to investigate novel genetic variations for the genetic improvement of crops. Maize is one of the most important cereal crops and provides daily calories to millions of people all over the world. This study aimed to investigate the genetic diversity and population structure of 32 local maize genotypes collected from the Black Sea Region of Turkey using SSR markers. Molecular characterization was performed using the 14 most polymorphic primers that yielded a total of 42 bands. An average of 3 alleles per SSR primer was detected, and the number of alleles varied from 1 (phi022) to 6 (umc1571). The unweighted pair-group method with arithmetic means (UPGMA) clustering divided maize accessions into three main populations. According to Nei's genetic distances, DZ-M-145 (Corum) and DZ-M-20 (Trabzon) genotypes were the closest (0.03) genetically related populations, while DZ-M-68 (Artvin) and DZ-M-55 (Rize) were the most genetically distant (0.63) populations. The study identified molecular genetic diversity not mentioned for maize plants from the Black Sea. On this occasion, new opportunities have been created for hybrid maize products.Öğe Exploring the genetic diversity and population structure of Turkish laurel germplasm by the iPBS-retrotransposon marker system(Mdpi, 2019) Karık, Ünal; Nadeem, Muhammad Azhar; Habyarimana, Ephrem; Ercişli, Sezai; Yıldız, Mehtap; Yılmaz, Abdurrahim; Baloch, Faheem ShehzadLaurel is a medicinally important plant and is known to the world for its essential oil. Turkey is the main market in the laurel leaf trade by sharing about 90% of the world trade. Here we made an effort to elucidate genetic diversity and population structure of 94 Turkish laurel genotypes collected from 26 provinces and four geographical regions using inter-primer binding site (iPBS) retrotransposon markers. A total of 13 most polymorphic primers were selected which yielded 195 total bands, of which 84.10% were found polymorphic. Mean polymorphism information content (PIC) was (0.361) and diversity indices including mean effective number of alleles (1.36), mean Shannon's information index (0.35) and overall gene diversity (0.22) revealed the existence of sufficient amount of genetic diversity in the studied plant material. Most diversity was found in genotypes collected from the Mediterranean region. Analysis of molecular variance (AMOVA) revealed that most of the variation (85%) in Turkish laurel germplasm is due to differences within populations. Model-based structure, principal coordinate analysis (PCoA) and neighbor-joining algorithms were found in agreement and clustered the studied germplasm according to their collection provinces and regions. This is a very first study exploring the genetic diversity and population structure of laurel germplasm using iPBS-retrotransposon marker system. We believe that information provided in this work will be helpful for the scientific community to take more interest in this forgotten but the medicinally important plant.Öğe Genetic characterization of rheum ribes (wild rhubarb) genotypes in lake Van basin of Turkey through Issr and ssr markers(Friends Science Publ, 2019) Ekincialp, Aytekin; Erdinç, Çeknas; Turan, Sibel; Çakmakçı, Özlem; Nadeem, Muhammad Azhar; Baloch, Faheem Shehzad; Şensoy, SuatRheum ribes L. (wild rhubarb) is one of the less known plants to the world and the only species from the Rheum genus present in Turkey. In this study, one R. rhabarbarum (as check genotype) and 80 R. ribes genotypes were collected from different geographical locations of Turkey for the investigation of diversity and genetic structure using ISSR (Inter Simple Sequence Repeat) and SSR (Simple Sequence Repeats) markers. SSR markers reflected higher (100%) polymorphism as compared to the ISSR marker. However, ISSR markers produced higher average Polymorphism Information Content (PIC) value (0.805) than the SSR markers (0.724). A Similar range of (PIC) values with ISSR markers was found greater (0.935-0.395) as compared to the range of SSR makers (0.88-0.47). Using Jaccard similarity index, genetic distance was measured for both markers and average genetic distance was found to be higher with ISSR markers as compared to the SSR markers. Neighbor-joining analysis clustered genotypes into 3 groups for both marker systems. During this study some distinct genotypes like R. rhabarbarum, YYUERC19, YYUERCO9 and YYUNIER65 were investigated that can be used as candidate parents for the development of R. ribes L. varieties. Structure analysis grouped the genotypes according to altitude by clustering genotypes having at more than 2000 m in one group and genotypes less than 2000 m altitude in another group. Genetic variations observed in this study can be applied to investigate various traits of interest for the R. ribes L. breeding. (C) 2019 Friends Science PublishersÖğe Genetic diversity analysis in the Turkish pepper germplasm using iPBS retrotransposonbased markers(2020) Yıldız, Mehtap; Koçak, Metin; Nadeem, Muhammad Azhar; Cavagnaro, Pablo; Barboza, Karina; Baloch, Faheem Shahzad; Argün, Duygu; Keleş, DavutCapsicum is one of the most important and diverse plant taxa, widely used as a spice and vegetable worldwide, including Turkey. Germplasm characterization is an essential step for crop breeding. In the present study, we characterized the genetic diversity and population structure of a collection of 94 pepper accessions using inter-primer binding site (iPBS) retrotransposon-based markers. A total of 20 iPBS primers were used that generated 172 bands (mean = 8.6 bands/primer), of which ~92% were polymorphic in the entire germplasm collection, whereas 83%, 69%, and 80% of the bands were polymorphic within the C. annuum, C. chinense, and C. frutescens subsets, respectively. All of the taxa analyzed were clearly differentiated by the iPBS markers. The polymorphism information content of the markers ranged between 0.15 and 0.99, with an average of 0.66. Cluster analyses by different methods (UPGMA, STRUCTURE, and principal coordinate analysis) revealed a clear separation of all of the C. annuum accessions from the other pepper species, with a few subclusters observed among the latter, including groups with accessions of both C. frutescens and C. chinense. At the interspecies level, the 3 clustering methods clearly discriminated C. annuum from C. frutescens and C. chinense. No clear association was found between the iPBS-based clustering and geographical origin or fruit characteristics of the accessions. This is the first report characterizing the genetic diversity and population structure in the Turkish pepper germplasm using iPBS markers. It is expected that these data will serve as a foundation for the development of new and improved pepper varieties.Öğe Genetic diversity and population structure of endangered rosewood from the Peruvian Amazon using ISSR markers(Inst Nacional Pesquisas Amazonia, 2020) Vasquez Guizado, Stalin Juan; Nadeem, Muhammad Azhar; Ali, Fawad; Barut, Muzaffer; Habyarimana, Ephrem; Pacheco Gomez, Tedi; Vargas Santillan, Jhon Antoni; Baloch, Faheem ShehzadRosewood, Aniba rosaeodora is an endangered species in Amazon forests and its natural stands have been heavily depleted due to over-exploitation for the cosmetic industry. This study aimed to investigate the genetic diversity and population structure of 90 rosewood accessions from eight localities in the Peruvian Amazon through 11 Inter Simple Sequence Repeats (ISSR) primers. The ISSR primers produced a sum of 378 bands, of which 375 (99.2%) were polymorphic, with an average polymorphism information content (PIC) value of 0.774. The mean effective number of alleles (Ne), Shannon informative index (I), gene diversity (He) and total gene diversity (Ht) were 1.485, 0.294, 0.453 and 0.252, respectively. Analysis of molecular variance (AMOVA) showed the presence of maximum variability within populations (88%). The Structure algorithm, neighbor joining and principal coordinate analysis (PCoA) grouped the 90 rosewood accessions into three main populations (A, B and C). Diversity indices at the inter-population level revealed a greater genetic diversity in population A, due to higher gene flow. The neighbor-joining analysis grouped populations A and B, while population C was found to be divergent at the inter population level. We concluded that population A reflects higher genetic diversity and should be prioritized for future management and conservation plans.Öğe Genetic diversity assessment in Nicotiana tabacum L. with iPBS-retrotransposons(2018) Yaldız, Gülsüm; Çamlıca, Mahmut; Nadeem, Muhammad Azhar; Nawaz, Muhammad Amjad; Baloch, Faheem ShehzadKnowledge of genetic diversity could be beneficial by contributing important information in the selection of breeding material. The objective of this study was to explore the genetic diversity and relationship in the Turkish tobacco germplasm with the iPBSretrotransposons marker that emerged as a universal method. A total of 90 landraces and 6 commercial cultivars collected from different geographical regions of Turkey were used in this study. Eleven most polymorphic iPBS-retrotransposons primers yielded a total of 119 scorable bands and 98 of these bands were found to be polymorphic (82.35%), with an average of 8.91 polymorphic fragments for each primer. The mean polymorphism information contents were found to be 0.33, ranging from 0.10 to 0.80. The unweighted pair group method with arithmetic mean revealed that the genotypes belonging to the same geographical regions were often present in the same group and very close to each other. Based on the Bayesian clustering model, the genetic structure of Turkish tobacco germplasm was divided into 2 main groups. This is first study to explore the genetic diversity of Turkish tobacco by the iPBS-retrotransposons and we believe that in future the results of this study will serve as a foundation for the development of new and improved tobacco varieties in Turkey and the rest of world.Öğe Genetic diversity, population structure and marker- trait association for 100-seed weight in international safflower panel using silicodart marker information(MDPI AG, 2020) Ali, Fawad; Nadeem, Muhammad Azhar; Barut, Muzaffer; Habyarimana, Ephrem; Sameeullah, Muhammad; Shehzad Baloch, FaheemSafflower is an important oilseed crop mainly grown in the arid and semi-arid regions of the world. The aim of this study was to explore phenotypic and genetic diversity, population structure, and marker-trait association for 100-seed weight in 94 safflower accessions originating from 26 countries using silicoDArT markers. Analysis of variance revealed statistically significant genotypic effects (p < 0.01), while Turkey samples resulted in higher 100-seed weight compared to Pakistan samples. A Constellation plot divided the studied germplasm into two populations on the basis of their 100-seed weight. Various mean genetic diversity parameters including observed number of alleles (1.99), effective number of alleles (1.54), Shannon’s information index (0.48), expected heterozygosity (0.32), and unbiased expected heterozygosity (0.32) for the entire population exhibited sufficient genetic diversity using 12232 silicoDArT markers. Analysis of molecular variance (AMOVA) revealed that most of the variations (91%) in world safflower panel are due to differences within country groups. A model-based structure grouped the 94 safflower accessions into populations A, B, C and an admixture population upon membership coefficient. Neighbor joining analysis grouped the safflower accessions into two populations (A and B). Principal coordinate analysis (PCoA) also clustered the safflower accessions on the basis of geographical origin. Three accessions; Egypt-5, Egypt-2, and India-2 revealed the highest genetic distance and hence might be recommended as candidate parental lines for safflower breeding programs. The mixed linear model i.e., the Q + K model, demonstrated that two DArTseq markers (DArT-45483051 and DArT-15672391) had significant association (p < 0.01) for 100-seed weight. We envisage that identified DArTseq markers associated with 100-seed weight will be helpful to develop high-yielding cultivars of safflower through marker-assisted breeding in the near future.Öğe Genomics, phenomics, and next breeding tools for genetic improvement of safflower (Carthamus tinctorius L.)(Springer International Publishing, 2021) Yilmaz, Abdurrahim; Yeken, Mehmet Zahit; Ali, Fawad; Barut, Muzaffer; Nadeem, Muhammad Azhar; Yilmaz, Hilal; Naeem, MuhammadSafflower is one of the most important oilseed crops with high-quality seed oil. It can be grown especially in the arid and semiarid regions in the world. The main reason why safflower is not widely cultivated is because of its low yield. Various breeding efforts have made a significant contribution to the improvement of safflower; however, it seems necessary to exploit the potentiality of this underutilized plant. The development of new and improved safflower varieties will improve the sustainability of this crop to different environmental conditions. Classic breeding efforts made great efforts in safflower breeding; however, these techniques have been slow to develop complex traits such as yield, oil yield, some quality traits, and biotic-abiotic stress resistance. Recent advancements in molecular markers and genome sequencing technologies enhanced the breeding activities and aided the scientific community to understand and comprehensively explore the genetic diversity and population structure of safflower. Phenotypic and molecular characterization helped the construction of genetic linkage maps, leading to a better understanding of complex quantitative characters for safflower varieties. The present chapter articulates different aspects of safflower including phenomics, chemical content, origin-diffusion, similarity centers, wild relatives, genetic resources, trades, and comprehensive advancement in safflower breeding in terms of classical breeding, tissue culture, QTL mapping, association mapping, transgenic breeding, genome editing, and speed breeding. This information will lead to more short-term solutions in breeding safflower crop and will provide more practical information for breeders in the near future. © Springer Nature Switzerland AG 2021.Öğe Investigation of Genetic Diversity in Lentil Genotypes Obtain from Different Countries Using SDS-PAGE Methodology(2024) Yeken, Mehmet Zahit; Baloch, Faheem Shehzad; Nadeem, Muhammad Azhar; Sameeullah, Muhammad; Gürel, EkremIn the current study, the characterization of 44 lentil genotypes was performed using SDS-PAGE (Sodium dodecyl sulfate-polyacrylamide gel electrophoresis) technique. The protein bands were scored according to a binary system as the present (1) or absent (0) that ranged 12-20 bands in lentil genotypes. The Jaccard’s coefficient of genetic dissimilarity (GD) was measured, and the mean GD was 0.216. Maximum genetic distance was found as 0.526 between Syria2 with Iraq3 and these genotypes might be recommended for future lentil breeding. AMOVA explored the presence of higher genetic variety within genotypes (83%) than among genotypes (17% variations). STRUCTURE algorithm separated lentil genotypes into two groups mainly on the basis of their geographic. The UPGMA clustering separated lentil genotypes into two main clusters A and B. The PCoA was also conducted to confirm the results of structure and UPGMA analyses. Findings from the PCoA and structure analyses were in full agreement with those obtained by UPGMA. The results might be useful for researchers worldwide who are interested in lentil breeding.Öğe Investigation of morphoagronomic performance and selection indices in the international safflower panel for breeding perspectives(2020) Ali, Fawad; Yılmaz, Abdurrahim; Chaudhary, Hassan Javed; Nadeem, Muhammad Azhar; Rabbani, Malik Ashiq; Arslan, Yusuf; Baloch, Faheem ShehzadDeveloping high yielding safflower cultivars with good adaptation to diverse environmental conditions can improve production in terms of seed yield and reduce the deficiency in edible oil. The genetic variability that exists among and within populations for desirable agronomic traits can be used to develop elite cultivars. A total of 94 safflower accessions from 26 different countries were used in this study to evaluate morphoagronomic performance, determine the pattern of similarity centers, and identify the best performing accessions by conducting 2 field experiments in Pakistan and Turkey using augmented design. Genetic diversity for important yield and yield traits was described including capitulum diameter (17.30 to 28.30 mm), branches per plant (5.10 to 17.30), capitula per plant (8.70 to 80.40), and seed yield per plant (4.86 to 51.02 g). These analyses showed a good level of variation in the current study. Using principal component analysis, it was observed that days to flower initiation, days to 50% flowering, days to flower completion, seed yield per plant, capitula per plant, branches per plant, seeds per capitulum, and capitulum diameter were the major contributors to the observed genetic variability in the evaluated safflower panel. Seed yield per plant reflected a significant and positive correlation with capitula per plant, branches per plant, and capitulum diameter, and these traits can be suggested as a selection criterion in safflower breeding programs. The hierarchical clustering was in agreement with the patterns of 7 similarity centers based on seed yield per plant, capitula per plant, capitulum diameter, and branches per plant. During this study, a few promising safflower accessions were selected for future breeding programs.Öğe Laurel (Laurus nobilis L.): A less-known medicinal plant to the world with diffusion, genomics, phenomics, and metabolomics for genetic improvement(Springer Singapore, 2018) Nadeem, Muhammad Azhar; Aasim, Muhammad; Kırıcı, Saliha; Karık, Ünal; Nawaz, Muhammad Amjad; Yılmaz, Abdurrahim; Baloch, Faheem ShehzadMedicinal plants have gained the world’s attention due to their application in various ways. Laurel (Laurus nobilis. L) is a very important medicinal plant of the Mediterranean region. Traditionally this plant has been successfully used in medicine, and its essential oil has great importance. Genomics, breeding, and metabolomics of different crops have remained the main focus of researchers, which made this plant to less known to the world. Most of the researchers only worked about the essential oil and its antibacterial and antioxidant activities. However, still almost no work has been done about the breeding aspects of this important plant. The present review offers an overview about the origin, diffusion, genomics, phenomics, breeding, and metabolomics of laurel. This information would be very helpful for the researchers who are interested in the breeding of this plant. © Springer Nature Singapore Pte Ltd. 2018.