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  • Küçük Resim
    Öğe
    Cytotoxic, anti-inflammatory and antioxidant activities of four different extracts of Galega officinalis L (goat's rue)
    (Pharmacotherapy Group, 2016) Karakaş, Fatma Pehlivan; Türker, Arzu Uçar; Karakaş, Alper; Mshvildadze, Vakhtang
    Purpose: To evaluate the cytotoxic, anti-inflammatory and antioxidant activities of four different solvent extracts obtained from the aerial parts of Galega officinalis L. Methods: The hexane, DCM, methanol and water extracts of G. officinalis were successively obtained by soxhlet extraction method. The cytotoxic activity of the extracts was assessed against human lung carcinoma (A-549), human colorectal adenocarcinoma (HT-29), human brain glioblastoma (U-87), and colon adenocarcinoma (DLD-1) by Resazurine test. The antioxidant activity of extracts were determined by Folin-Ciocalteau, oxygen radical absorbing capacity (ORAC), and 2'.7'-dichlorofluorescin-diacetate (DCFH-DA) cell-based assay while their anti-inflammatory activity was determined by nitric oxide (NO) assay. Results: DCM extract showed strong cytotoxic activity against lung adenocarcinoma and brain glioblastoma cell lines, with IC50 (concentration inhibiting 50 % of cell growth) values of 11 +/- 0.4 and 16 +/- 3 mu g/mL, respectively. The hexane extract showed moderate anticancer activity against the same cell lines (59 +/- 13 and 63 +/- 16 mu g/mL, respectively). DCM extract also showed significant anti-inflammatory activity, inhibiting NO release by 86.7 % at 40 mu g/mL in lipopolysaccharide (LPS) - stimulated murine RAW 264.7 macrophages. Of all test extracts, the methanol extract of G. officinalis showed the highest antioxidant activity with 2.33 +/- 0.09 mu mol Trolox/mg, 7.10 +/- 0.9 g tannic acid equivalent (TAE), and IC50 of 44 +/- 4 mu g/mL. Conclusion: The findings of this study suggest that DCM extract may possess anticancer effect against lung adenocarcinoma and brain glioblastoma, as well as serve as an anti-inflammatory agent.
  • Küçük Resim
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    In vitro cytotoxic, antibacterial, anti-inflammatory and antioxidant activities and phenolic content in wild-grown flowers of common daisy-A medicinal plant
    (Elsevier Gmbh, Urban & Fischer Verlag, 2017) Karakaş, Fatma Pehlivan; Türker, Arzu Uçar; Karakaş, Alper; Mshvildadze, Vakhtang; Pichette, Andre
    Bellis perennis L. is a medicinal plant in the family Compositae. It has been used as a remedy for wounds, rheumatism, eczema, eye diseases, inflammation and tonsillitis in folk medicine. In the present study, 19 different extracts and two fractions were obtained from wild-grown flowers, leaves and/or in vitro-grown leaves of common daisy by using different solvents and extraction methods. Biological activities of these extracts and fractions were assessed using selected bioassays: cytotoxic activity, disc diffusion assay, radical scavenging activity (DPPH), total phenolic content, oxygen radical absorbing capacity (ORAC) and 20,70-dichlorofluorescin-diacetate (DCFH-DA) cell-based assays. The cytotoxic activity of extracts and fractions was investigated against human lung carcinoma (A-549) and colon adenocarcinoma (DLD-1) cells. In vitro-grown leaf extracts showed the highest cytotoxic activity against selected cell lines. Moreover, n-butanol (n-BuOH) and ethyl acetate (EtOAc) fractions of flowers exerted high levels of cytotoxic activity. The MeOH extract and the EtOAc fraction of flowers exhibited broad-spectrum antibacterial activity against Streptococcus pyogenes, Staphylococcus aureus, Staphylococcus epidermidis, and Enterobacter cloacea. The strongest antioxidant activity was found in the EtOAc fraction of flowers with the highest amount of phenolic content and ORAC value. The MeOH extract of flowers showed strong anti-inflammatory activity on RAW 264.7 macrophages. The amount of the chosen 22 phenolic compounds in dichloromethane (DCM), MeOH extracts, n-BuOH and EtOAc fractions of field-grown flowers was detected using LC-ESI-MS/MS. The results of these studies support the potential use of B. perennis for wounds, rheumatism, inflammation, cancer and eye diseases. (C) 2016 Elsevier GmbH. All rights reserved.