Yazar "Le, Bao" seçeneğine göre listele

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  • Küçük Resim
    Öğe
    Development of a competent and trouble free dna isolation protocol for downstream genetic analyses in glycine species
    (2016) Nawaz, Muhammad Amjad; Baloch, Faheem Shehzad; Rehman, Hafiz Mamoon; Le, Bao; Akther, Fahima
    Extraction of deoxyribose nucleic acid (DNA) from plants is preliminary step in molecular biology. Fast and cost effective genomic DNA isolation from Glycine species for downstream application is a major bottleneck. Here we report a high throughput and trouble free method for genomic DNA extraction from leaf and seeds of Glycine species with high quality and quantity. Protocol reports the optimization by employing different concentrations of CTAB and PVP in extraction buffer. Efficiency of optimized protocol was compared with frequently used DNA extraction methods. Wide adoptability and utility of this protocol was confirmed by DNA extraction from leaves as well as seeds of G. max, G. soja, G. tomentella and G. latifolia. Extracted DNA was successfully subjected to PCR amplification of five microsatellite markers and four putative glycosyltransferase genes. DNA extraction protocol is reproducible, trouble free, rapid and can be adopted for plant molecular biology applications.
  • Küçük Resim Yok
    Öğe
    Development of a Competent and Trouble Free DNA Isolation Protocol for Downstream Genetic Analyses in Glycine Species
    (2016) Nawaz, Muhammad Amjad; Baloch, Faheem Shehzad; Rehman, Hafiz Mamoon; Le, Bao; Akther, Fahima; Yang, Seung Hwan; Chung, Gyuhwa
    Extraction of deoxyribose nucleic acid (DNA) from plants is preliminary step in molecular biology. Fast and cost effective genomic DNA isolation from Glycine species for downstream application is a major bottleneck. Here we report a high throughput and trouble free method for genomic DNA extraction from leaf and seeds of Glycine species with high quality and quantity. Protocol reports the optimization by employing different concentrations of CTAB and PVP in extraction buffer. Efficiency of optimized protocol was compared with frequently used DNA extraction methods. Wide adoptability and utility of this protocol was confirmed by DNA extraction from leaves as well as seeds of G. max, G. soja, G. tomentella and G. latifolia. Extracted DNA was successfully subjected to PCR amplification of five microsatellite markers and four putative glycosyltransferase genes. DNA extraction protocol is reproducible, trouble free, rapid and can be adopted for plant molecular biology applications.
  • Küçük Resim
    Öğe
    Isoflavone profile diversity in Korean wild soybeans (Glycine soja Sieb. & Zucc.)
    (2018) Tsukamoto, Chigen; Nawaz, Muhammad Amjad; Kurosaka, Ayaka; Le, Bao; Lee, Jeong Dong; Baloch, Faheem Shehzad
    Isoflavones prevent the incidence of cancer, reduce cardiovascular problems, and decrease menopausal symptoms. In soybean (Glycine soja Sieb. & Zucc.) seeds, the content is greatly affected by growing conditions. The variations in soy-isoflavone content were investigated by growing 346 Korean wild soybean accessions from natural habitats under the same agroclimatic conditions. Highperformance liquid chromatography was used to measure the profiles of six soy-isoflavones, i.e. glycosides (daidzin, glycitin, genistin) and malonyl glycosides (malonyl-daidzin, malonyl-glycitin, and malonyl-genistin). The soy-isoflavone content of the wild soybeans ranged from 0.5 to 5.51 mg/g with an average of 2.78 mg/g. The highest mean total soy-isoflavone was genistin (1.393 mg/g), followed by daidzin (0.939 mg/g) and glycitin (0.250 mg/g). Korean wild soybean seeds examined in this study were rich in malonyl-genistin. An increasing trend for total soy-isoflavone content was observed from the north to the southeast in the geographical distribution sites, which signifies the effect of genotype and collection site. The information generated in this report will greatly aid soybean breeding stratagems aimed at improving soy-isoflavone concentration in soybean seeds.