Yazar "Konaç, Ece" seçeneğine göre listele

Listeleniyor 1 - 4 / 4
  • Küçük Resim
    Öğe
    Angiopoietin-2 Gene Polymorphisim in Sporadic Prostate Cancer
    (2007) Önen, Hacer İlke; Konaç, Ece; Eroğlu, Muzaffer; Emekci, Abdullah
    Amaç: Anjiyogenezis, prostat kanserinin lokal ilerleyişi ve metastazında kritik bir role sahiptir. Anjiyogenezise bağımlı genlerde meydana gelen tek nükletit polimorfizmleri, kanserin oluşum ve ilerleme derecesini etkilemektedirler. Anjiyopoietin-2 (ANGPT-2), vasküler büyüme ve regresyonun en önemli düzenleyicilerinden olmakla beraber, prostat ve prostat tümörlerindeki rolü bilinmemektedir. ANGPT-2’de var olan DNA sekans varyasyonları, genlerin ürünlerini veya aktivitelerini değiştirebilirler. Bu çalışmada ANGPT-2 exon 4 G/A’da meydana gelen değişimlerin Türk popülasyonunda sporadik prostat kanser hastalarını nasıl etkilediğini belirlemeyi amaçladık. Gereç ve Yöntemler: 52 sporadik prostat kanser hastası ve 52 sağlıklı kontrolü kapsayan hasta kontrol çalışması yapılmıştır. Farklı ANGPT-2 alellerini tanımlamak amacıyla, Polimeraz Zincir Reaksiyonu (PCR) ve Restriksiyon Parçacık Uzunluk Polimorfizmi (RFLP) yöntemleri uygulanmıştır. Bulgular: Polimorfizme ait genotip ve alel frekans dağılımları hastalar ve kontrol grubu arasında istatistiksel olarak anlamlı bir farklılığa işaret etmemiştir (P>0.05). Ayrıca, hastaların tümör lenf nodu metastazı (TNM), Gleason skoru (GS) ve serum prostat-spesifik antijeni (PSA) seviyelerine göre sınıflandırılması, ANGPT-2 exon 4 G/A genotipleri arasında anlamlı farklılıklar göstermemiştir (P>0.05). Sonuç: Bu çalışma, sporadik prostat kanseri hastalarında ANGPT-2 exon 4 G/A polimorfizmi üzerine yapılan ve bu polimorfizmin Türk popülasyonunda prostat kanseri ile ilişkili olmadığını gösteren ilk çalışmadır.
  • Küçük Resim Yok
    Öğe
    Angiopoietin-2 gene polymorphism in sporadic prostate cancer
    (2007) Önen, H. Ilke; Konaç, Ece; Ero?lu, Muzaffer; Ekmekci, Abdullah
    Purpose: Angiogenesis is a critical requirement for local proliferation and metastasis of prostate cancer. Single nucleotide polymorphisms in angiogenesis-dependent genes affect the degree of cancer development and progression. Angiopoietin-2 (ANGPT-2) is one of the principal regulators of vascular growth and regression; however, its role in normal prostate and prostate tumors is largely unknown. DNA sequence variations in ANGPT-2 may alter the production outcomes or activities of the genes. In this study, we aimed to determine how the changes in the ANGPT-2 exon 4 G/A affect sporadic prostate cancer patients in the Turkish population. Materials and Methods: A case-control study on 52 sporadic prostate cancer patients and 52 healthy control subjects was conducted. Polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) analyses were performed to identify different ANGPT-2 alleles. Results: The distribution of genotype and allele frequencies of the polymorphism did not yield a statistically significant difference between patients and controls (P>0.05). Furthermore, classification of patients by tumor, lymph nodes, metastasis (TNM), Gleason scores (GS), and serum prostate-specific antigen (PSA) levels showed no significant differences among the ANGPT-2 exon 4 G/A genotypes (P>0.05). Conclusion: This is the first report on the ANGPT-2 exon 4 G/A polymorphism in patients with sporadic prostate cancer demonstrating that the investigated polymorphism is not associated with prostate cancer in the Turkish population.
  • Küçük Resim Yok
    Öğe
    Association of Genetic Polymorphisms in Vitamin D Receptor Gene and Susceptibility to Sporadic Prostate Cancer
    (Sage Publications Ltd, 2008) Önen, İlke Hacer; Ekmekçi, Abdullah; Eroğlu, Muzaffer; Konaç, Ece; Yeşil, Süleyman
    Genetic and environmental factors are involved in prostate cancer (PCa) etiology. Single nucleotide polymorphisms (SNPs) may contribute to the PCa pathogenesis. The goal of this study is to determine the role of vitamin D receptor (VDR) gene polymorphisms and haplotypes in the development and progression of sporadic PCa. One hundred and thirty-three PCa patients and 157 age-matched healthy controls were genotyped for the ApaI (rs7975232), BsmI (rs1544410) and TaqI (rs731236) polymorphisms in VDR gene by using polymerase chain reaction-restriction fragment length polymorphism. An association was observed between the ApaI polymorphism and PCa predisposition (P = 0.03). When compared with AA genotype, there was a highly notable difference in the frequencies of the Aa (P = 0.02), aa (P = 0.026) and ApaI "a" allele carriers (Aa + aa) (P = 0.009) genotypes. Furthermore, we found a statistical difference in the allele frequencies of the ApaI polymorphism between the sporadic PCa patients and control subjects (P = 0.013). The genotype distribution for the BsmI and TaqI polymorphisms were similar between cases and controls (P > 0.05). No clinically significant relationship was found between the three-locus haplotypes and development of sporadic PCa. The genotype frequencies for the three polymorphisms of the VDR gene within subgroups of PCa (defined by tumor stage, Gleason score, PSA levels) were also analyzed, but no statistically noteworthy difference was observed (P > 0.05). As far as we know, this is the first study which investigates the relationship between VDR genotypes and sporadic PCa in the Turkish population. Our findings suggest that the VDR ApaI (rs7975232) polymorphism may play a role in the development of sporadic PCa. Exp Biol Med 233:1608-1614, 2008
  • Küçük Resim
    Öğe
    No association between polymorphism in the vascular endothelial growth factor gene at position-460 and sporadic prostate cancer in the Turkish population
    (Springer, 2008) Önen, İlke H.; Konaç, Ece; Eroğlu, Muzaffer; Güneri, Çağrı; Biri, Hasan; Ekmekçi, Abdullah
    The development and progression of prostate cancer (PCa) has biologically and genetically remained a mystery. A man's risk of developing PCa is influenced by both genetic and environmental factors. Angiogenic cytokines like vascular endothelial growth factor (VEGF) play a pivotal role in tumor angiogenesis. Single nucleotide polymorphisms in angiogenesis-dependent genes affect the sensibility of cancer development and progression. Therefore, we hypothesized a potential association between DNA sequence variations in VEGF -460 gene region and sporadic PCa patients in the Turkish population. 133 sporadic PCa patients and 157 healthy controls were studied. Genotypes were determined by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) analysis. The distribution of genotype and allele frequencies of the polymorphism did not yield a statistically significant difference between patients and controls (P > 0.05). Furthermore, classification of patients by tumor-lymph nodes-metastasis (TNM), Gleason Scores (GS) and serum prostate-specific antigen (PSA) levels did not show significant differences among the VEGF -460 C > T genotypes (P > 0.05). This is the first demonstration showing that the VEGF -460 C > T polymorphism in men is not associated with sporadic PCa in the Turkish population.