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Öğe Inter-primer binding site retrotransposon and inter-simple sequence repeat diversity among wild Lens species(Pergamon-Elsevier Science Ltd, 2015) Baloch, Faheem Shehzad; Derya, Muazzez; Andeden, Enver Ersoy; Alsaleh, Ahmad; Cömertpay, Gönül; Kilian, Benjamin; Özkan, HakanEven though lentil has been an important food legume for centuries, genetic studies in lentil are still in their infancy. Genetic diversity and relationships among wild Lens species from Turkey has seldom been investigated. Additionally, a limited number of simple sequence repeat (SSR) markers have been developed for use in breeding and genetic studies of lentil crop. In this study, molecular characterization of 50 accessions mostly from Turkey, belonging to 6 wild and 1 cultivated Lens species, was performed using newly developed inter-primer binding site (iPBS) retrotransposons and inter-SSR (ISSR) markers. The 10 iPBS primers generated a total of 151 scorable bands, of which 150 were polymorphic (99.3%) with an average of 15.0 polymorphic fragments per primer. The 10 ISSR primers detected 138 scorable bands showing 100% polymorphism, with an average of 13.5 bands per primer. The average polymorphism information content (PIC) value for ISSR markers (0.97) was higher than that for iPBS markers (0.90). Lens orientalis was found to be the most diverse species, raising the possibility of wide crosses with cultivated species Lens culinaris. Cultivated varieties also showed high level of polymorphism, at 82.92% and 51.92% with ISSR and iPBS markers, respectively. Lens lamottei and Lens tomentosus were found as the least polymorphic species using both marker systems. The grouping of accessions and species within clusters were almost similar when iPBS and ISSR graphs were compared. Our data also suggested the role of iPBS-retrotransposons as 'a universal marker' for molecular characterization of wild and cultivated Lens species. (C) 2014 Elsevier Ltd. All rights reserved.Öğe Mirza (Hacızade) Gökgöl (1897-1981): the great explorer of wheat genetic resources in Turkey(Springer, 2018) Zencirci, Nusret; Yılmaz, Hande; Garaybayova, Nargiz; Karagöz, Alptekin; Kilian, Benjamin; Özkan, Hakan; Hammer, KarlMirza (Hacızade) Gökgöl (1897-1981) was a leading agro-ecological plant scientist, agronomist and breeder in Turkey. Gokgol carried out extensive breeding programmes extensively selecting from local plant genetic resources from across Turkey from 1925 till the 1950s. He collected and evaluated cultivars, landraces and wild relatives, predominantly of wheat, but also of barley, lentil and other crops in Turkey during 1929-1955. He published books on wheat and other cereals, potatoes, castor bean, sweet clover, poppy, and other crops. He was a hardworking pioneer utilizing wheat genetic resources for crop improvement. Since his ample work has hardly been accessible by the English-speaking scientific community today, we are providing an overview of his major work, mainly on wheat. The books he published can still be found in libraries, although with difficulties. However, his articles, leaflets etc., are hardly accessible. Some of them are: Turkish Wheats, vols. 1 and 2, Plant Breeding through Scientific Methods (1928, translation of a book by E. Baur), The Basics of Wheat Breeding (1954), and Agriculture and Breeding of Cool Season Cereals-Wheat, Rye, Barley, and Oats (1969). In addition to the summary of his books, we reviewed his views and discussions about the centres of origin and diversity, and compared with those of Vavilov.