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  • Küçük Resim
    Öğe
    Effects of erdosteine on alpha amanitin-induced hepatotoxicity in mice
    (Taylor and Francis Ltd, 2016) Kaya, Ertugrul; Yılmaz, İsmail; Admış, Özlem; Oktay, Murat; Bayram, Recep
    The aim of this study was to investigate beneficial effects of erdosteine in the alpha amanitine-induced hepatotoxicity in mice. Three hours after giving alpha amanitin (0.5 mg/kg, i.p.) to the mice, they were administered silibinin (50 mg/kg/d, i.p.) or erdosteine (100 mg/kg/d, oral) therapies once a day for 3 d. A histopathological examination of their liver tissues was carried out 24 h after the last treatment; transaminase levels, blood urea nitrogen, urea, and creatinine were analyzed in serum. Erdosteine showed a beneficial effect by significantly improving the functional parameters particularly in alpha amanitin-induced hepatotoxicity and partially in renal toxicity. In the histopathological evaluation, the toxicity that was generated with alpha amanitin was significantly reduced by erdosteine, showing a possible hepatoprotective effect. © 2016 Informa UK Limited, trading as Taylor & Francis Group.
  • Küçük Resim Yok
    Öğe
    Erdosteine reduces cytotoxicity induced by alpha- and beta-amanitin, but not gamma-amanitin, in CA3 hepatocyte cultures
    (Pergamon-Elsevier Science Ltd, 2022) Bayram, Recep; Yilmaz, Ismail; Yaykasli, Kursat Oguz; Kaya, Ertugrul
    Amanitin poisoning still has no particular, effective antidote. Erdosteine has been shown to protect numerous tissues, particularly those in the liver. This study investigates the potential therapeutic effects of erdosteine on alpha-, beta-and gamma-amanitin-induced hepatotoxicity in in vitro models. Three hours after administering amatoxins at various concentrations (1-50 mu g/mL) to the cells of the C3A human hepatocyte cell line, erdosteine was administered in different concentrations (i.e., 1, 10, 50, 100 and 250 mu g/mL). The 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) assay was selected to determine cell viability. When concentrations of 1, 10, 50, 100 and 250 mu g/mL of erdosteine were applied to cell lines, the following cell viability rates were obtained: 106%,99%,93%,86% and 86%, respectively, at a 10 mu g/mL alpha-amanitin-induced toxicity; 43%,41%,41%,37% and 35%, respectively, at a 25 mu g/mL alpha-amanitin-induced toxicity; 44%,42%,41%,39% and 41%, respectively, at a 50 mu g/mL alpha-amanitin-induced toxicity; 136%,142%,143%,137% and 120%, respectively, at a 10 mu g/mL beta-amanitin-induced toxicity; 113%,107%,107%,106% and 86%, respectively, at a 25 mu g/mL beta-amanitin-induced toxicity; 78%,77%,77%,74% and 70%, respectively, at a 10 mu g/mL gammaamanitin-induced toxicity; and 39%,40%,39%,35% and 31%, respectively, at a 25 mu g/mL gamma-amanitininduced toxicity. This study was the first to evaluate the in vitro efficacy of erdosteine in cytotoxicity induced by alpha-, beta-and gamma-amanitin. Non-high (low and medium) doses of erdosteine are capable of nearly entirely preventing toxicity at mild hepatotoxic concentrations caused by amatoxin and partially preventing toxicity at moderate and severe concentrations. The beneficial effects of erdosteine, especially on the toxicity of alpha-and beta-amanitin, are promising.
  • Küçük Resim Yok
    Öğe
    Purification of High Purity Alpha Amanitin Using Preparative HPLC Method
    (Duzce Univ, 2012) Kaya, Ertugrul; Karahan, Selim; Yaykasli, Kursat Oguz; Bayram, Recep; Saritas, Ayhan
    Objective: Alpha-amanitin is purified only around 90% purity using existing methods. In this study, it was aimed to describe the method in order to obtain high-purity alpha-amanitin using preparative HPLC. Methods: Amanita phalloides mushroom was collected, extracted and purified 2 times using preparative HPLC. Validation of the toxin was performed by comparison of retention time and ultraviolet spectrum at HPLC. Results: Alpha-amanitin was obtained with 93% (+/- 1.24) purity after first purification process. Alpha-amanitin was obtained with 99,8% (+/- 0.26) purity after second purification process. It was seemed that purified toxin and standard were given maximum absorbance at 303 nm and minimum absorbance at 263 nm, and the structure of the spectrums for both was similar. Conclusion: Alpha-amanitin with >99% purity can be obtained by this method at low cost.