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  • Küçük Resim
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    Changes in total phenols, total flavonoids, and antioxidant activities of common beans and pinto beans after soaking, cooking, and in vitro digestion process
    (Korean Society Food Science & Technology-Kosfost, 2010) Akıllıoğlu, H. Gül; Karakaya, Sibel
    Even though bean varieties are widely consumed all over the world, data related to how cooking methods and in vitro digestion affect bioactive compounds they contain and data related to bioavailability of polyphenols are limited. The aim of the present study was to investigate how some cooking methods and in vitro digestion influence antioxidant activity, total phenols (TP), and total flavonoids (TF) of widely consumed beans in Turkey. Soaking caused a significant decrease (25.61-38.63%) in the bioavailability of TP of dry common beans (CB). Soaking in cold water resulted in a significant decrease in TP bioavailability of dry pinto beans (PB). TF content was well retained in PB cooked without soaking but was not detected in CB after in vitro digestion. CB soaked in hot water and cooked with the addition of NaHCO3 showed the greatest inhibition effect on 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical (p<0.05). In vitro digestion caused increase in the antioxidant activity of both CB and PB.
  • Küçük Resim
    Öğe
    Effects of heat treatment and in vitro digestion on the Angiotensin converting enzyme inhibitory activity of some legume species
    (Springer, 2009) Akıllıoğlu, H. Gül; Karakaya, Sibel
    In this study, potential Angiotensin Converting Enzyme (ACE) inhibitory activity of common dry beans, dry pinto beans and green lentils were determined and the effects of different heat treatment periods were investigated. In vitro gastrointestinal digestion was applied after heat treatment, and stability of the ACE inhibitory activity was determined. Crude protein extracts of legume samples showed 4.08-28.54% ACE inhibition activities. 30-min heat treatment caused a decrease in ACE inhibitory activity; however, 50-min heat treatment was observed to be beneficial for the release of ACE inhibitory peptides from the three legume species (p < 0.05). In vitro digestion process increased ACE inhibitory activities of the samples (p < 0.05), mainly green lentil digests by a factor of 10.91-23.65 against ACE in comparison with raw sample. It was observed that IC50 values of common beans, pinto beans and green lentils after digestion were 0.78-0.83, 0.15-0.69 and 0.008-0.89 mg protein/mL, respectively.